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  SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy.

Lukinavicius, G., Blaukopf, C., Pershagen, E., Schena, A., Reymond, L., Derivery, E., et al. (2015). SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy. Nature Communications, 6: 8497. doi:10.1038/ncomms9497.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0029-04AF-4 Version Permalink: https://hdl.handle.net/21.11116/0000-0002-A6E9-C
Genre: Journal Article

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http://www.nature.com/ncomms/2015/151001/ncomms9497/pdf/ncomms9497.pdf (Publisher version)
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 Creators:
Lukinavicius, G.1, Author           
Blaukopf, C., Author
Pershagen, E., Author
Schena, A., Author
Reymond, L., Author
Derivery, E., Author
Gonzalez-Gaitan, M., Author
D'Este, E.2, Author           
Hell, S. W.2, Author           
Gerlich, D. W., Author
Johnsson, K., Author
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              
2Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR–Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.

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Language(s): eng - English
 Dates: 2015-10-01
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/ncomms9497
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Title: Nature Communications
Source Genre: Journal
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Pages: 7 Volume / Issue: 6 Sequence Number: 8497 Start / End Page: - Identifier: -