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Free keywords:
Grey side-gilled sea slug; Pleurobranchaea maculata; Tetrodotoxin;
Microsatellite markers; 454 pyrosequencing
Abstract:
Using 454 pyrosequencing data, 24 polymorphic microsatellite markers were identified for
the grey side-gilled sea slug, Pleurobranchaea maculata. The grey side-gilled sea slug is
found throughout the western and south Pacific and is known to contain high concentrations
of tetrodotoxin. Polymorphism was assessed in 20 individuals obtained from
geographically distinct locations within New Zealand. Between 2 and 15 alleles were
identified at each locus. The observed heterozygosity (Ho) and expected heterozygosity
(He) ranged from 0.10 to 1 and 0.10e0.94, respectively. No significant linkage disequilibrium
between pairs of loci or deviations from the HardyeWeinberg proportions were
observed. The markers are central to understanding the population biology and genetic
structure of P. maculata.
