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  Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells

Henkel, A. W., Meiri, H., Horstmann, H., Lindau, M., & Almers, W. (2000). Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells. The EMBO Journal, 19, 84-93. doi:10.1093/emboj/19.1.84.

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Genre: Zeitschriftenartikel
Alternativer Titel : Rhythmic opening and closing of vesicles during constitutive exo- and endocytosis in chromaffin cells

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http://emboj.embopress.org/content/19/1/84.full.pdf (beliebiger Volltext)
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 Urheber:
Henkel, Andreas Wolfram1, Autor           
Meiri, H., Autor
Horstmann, Heinz2, Autor           
Lindau, Manfred3, Autor           
Almers, Wolfhard3, Autor           
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
3Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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Schlagwörter: chemical oscillator; membrane capacitance; rapid freezing; staurosporine
 Zusammenfassung: Constitutive exo- and endocytic events are expected to increase and diminish the cell surface area in small spontaneous steps. Indeed, cell-attached patch-clamp measurements in resting chromaffin cells revealed spontaneous upward and downward steps in the electrical capacitance of the plasma membrane. The most frequent step size indicated cell surface changes of <0.04 ?m2, corresponding to vesicles of <110 nm diameter. Often downward steps followed upward steps within seconds, and vice versa, as if vesicles transiently opened and closed their lumen to the external space. Transient openings and closings sometimes alternated rhythmically for tens of seconds. The kinase inhibitor staurosporine dramatically increased the occurrence of such rhythmic episodes by making vesicle closure incomplete and by inhibiting fission. Staurosporine also promoted transient closures of large endocytic vesicles possibly representing remnants of secretory granules. We suggest that staurosporine blocks a late step in the endocytosis of both small and large vesicles, and that endocytosis involves a reaction cascade that can act as a chemical oscillator

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Sprache(n): eng - English
 Datum: 2000-10-192000-11-102000-01-042000-12
 Publikationsstatus: Erschienen
 Seiten: 10
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
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Titel: The EMBO Journal
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Nature Publishing Group
Seiten: - Band / Heft: 19 Artikelnummer: - Start- / Endseite: 84 - 93 Identifikator: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061_1