Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

Bittmann, J., Grigaitis, R., Galanti, L., Amarell, S., Wilfling, F., Matos, J., et al. (2020). An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases. eLife, 9: e52459. doi:10.7554/eLife.52459.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Bittmann, Julia1, Autor
Grigaitis, Rokas1, Autor
Galanti, Lorenzo1, Autor
Amarell, Silas1, Autor
Wilfling, Florian2, Autor           
Matos, Joao1, Autor
Pfander, Boris1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Jentsch, Stefan / Molecular Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565156              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein functionality at different cell cycle phases. We apply this technology to the question of how and when Mus81-Mms4 and Yen1 nucleases act on DNA replication or recombination structures. Restriction of Mus81-Mms4 to M phase but not S phase allows a wildtype response to various forms of replication perturbation and DNA damage in S phase, suggesting it acts as a post-replicative resolvase. Moreover, we use cell cycle tags to reinstall cell cycle control to a deregulated version of Yen1, showing that its premature activation interferes with the response to perturbed replication. Curbing resolvase activity and establishing a hierarchy of resolution mechanisms are therefore the principal reasons underlying resolvase cell cycle regulation.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2019-10-042020-04-292020-05-01
 Publikationsstatus: Online veröffentlicht
 Seiten: 29
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000535191000001
DOI: 10.7554/eLife.52459
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: eLife
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Cambridge : eLife Sciences Publications
Seiten: - Band / Heft: 9 Artikelnummer: e52459 Start- / Endseite: - Identifikator: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X