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  Subclones of C6 rat glioma cells differing in intermediate filament protein expression

Röser, K., Bohn, W., Giese, G., & Mannweiler, K. (1991). Subclones of C6 rat glioma cells differing in intermediate filament protein expression. Experimental Cell Research, 197(2), 200-206. doi:10.1016/0014-4827(91)90423-R.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-62D2-4 Version Permalink: https://hdl.handle.net/21.11116/0000-0000-62D3-3
Genre: Journal Article

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ExpCellRes_197_1991_200.pdf (Any fulltext), 6MB
 
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 Creators:
Röser, Kerstin, Author
Bohn, Wolfgang, Author
Giese, Günter1, 2, Author           
Mannweiler, Klaus, Author
Affiliations:
1Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              
2Light Microscopy Facility, Max Planck Institute for Medical Research, Max Planck Society, ou_1497720              

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 Abstract: The C6 rat glioma cell line is shown to consist of a mixed population of cells which either contain vimentin (80% of the cells) or completely lack any cytoplasmic intermediate filament (IF) proteins. Subclones could be established with both phenotypes, indicating that these IF protein expression patterns represent stable phenotypic markers. Absence of IF proteins in C6 subclones could consistently be correlated with an altered cell morphology and a pronounced increase in the number of actin stress fibers. In vitro translation and hybridization assays suggest the absence of vimentin to result from a block at the transcriptional level. The data indicate that subcloning of the C6 cell line on the basis of IF protein expression seems to be a reasonable approach for obtaining homogeneous C6 cell populations which may represent suitable experimental models for studies on vimentin expression and glioma cell differentiation.

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Language(s): eng - English
 Dates: 1991-04-301991-07-302004-10-261991-12
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0014-4827(91)90423-R
URI: https://www.ncbi.nlm.nih.gov/pubmed/1720390
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Title: Experimental Cell Research
  Other : Exp. Cell Res.
Source Genre: Journal
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Publ. Info: San Diego, CA : Academic Press
Pages: - Volume / Issue: 197 (2) Sequence Number: - Start / End Page: 200 - 206 Identifier: ISSN: 0014-4827
CoNE: https://pure.mpg.de/cone/journals/resource/954922645016