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  Cell-accurate optical mapping across the entire developing heart

Weber, M., Scherf, N., Meyer, A. M., Panáková, D., Kohl, P., & Huisken, J. (2017). Cell-accurate optical mapping across the entire developing heart. eLife, 6: e28307. doi:10.7554/eLife.28307.001.

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Weber_Scherf_2017.pdf (Verlagsversion), 3MB
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 Urheber:
Weber, Michael1, 2, Autor
Scherf, Nico1, 3, Autor           
Meyer, Alexander M.4, Autor
Panáková, Daniela4, 5, Autor
Kohl, Peter6, Autor
Huisken, Jan1, 7, Autor
Affiliations:
1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany, ou_persistent22              
2Harvard Medical School, Boston, MA, USA, ou_persistent22              
3Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_2205649              
4Max Delbrück Center for Molecular Medicine, Berlin, Germany, ou_persistent22              
5German Centre for Cardiovascular Research, Berlin, Germany, ou_persistent22              
6Faculty of Medicine, Albert Ludwigs University Freiburg, Germany, ou_persistent22              
7Morgridge Institute for Research, Madison, WI, USA, ou_persistent22              

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 Zusammenfassung: Organogenesis depends on orchestrated interactions between individual cells and morphogenetically relevant cues at the tissue level. This is true for the heart, whose function critically relies on well-ordered communication between neighboring cells, which is established and fine-tuned during embryonic development. For an integrated understanding of the development of structure and function, we need to move from isolated snap-shot observations of either microscopic or macroscopic parameters to simultaneous and, ideally continuous, cell-to-organ scale imaging. We introduce cell-accurate three-dimensional Ca2+-mapping of all cells in the entire electro-mechanically uncoupled heart during the looping stage of live embryonic zebrafish, using high-speed light sheet microscopy and tailored image processing and analysis. We show how myocardial region-specific heterogeneity in cell function emerges during early development and how structural patterning goes hand-in-hand with functional maturation of the entire heart. Our method opens the way to systematic, scale-bridging, in vivo studies of vertebrate organogenesis by cell-accurate structure-function mapping across entire organs.

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Sprache(n): eng - English
 Datum: 2017-12-29
 Publikationsstatus: Online veröffentlicht
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.7554/eLife.28307.001
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Projektname : Cardiac connective tissue: Beat-by-beat relevance for heart function in health and disease / CARDIONECT
Grant ID : 323099
Förderprogramm : Funding Programme 7
Förderorganisation : European Commission (EC)
Projektname : -
Grant ID : VH-NG-736
Förderprogramm : Young Investigator Program
Förderorganisation : Helmholtz-Gemeinschaft

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Titel: eLife
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Cambridge : eLife Sciences Publications
Seiten: - Band / Heft: 6 Artikelnummer: e28307 Start- / Endseite: - Identifikator: ISSN: 2050-084X
CoNE: https://pure.mpg.de/cone/journals/resource/2050-084X