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Zusammenfassung:
A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell superresolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP-tag, together with a non-covalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.