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  Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement

Budkevich, T. V., Giesebrecht, J., Behrmann, E., Loerke, J., Ramrath, D. J., Mielke, T., et al. (2014). Regulation of the mammalian elongation cycle by subunit rolling: a eukaryotic-specific ribosome rearrangement. Cell, 158(1), 121-31. doi:10.1016/j.cell.2014.04.044.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0028-6375-0 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0028-6376-E
Genre: Journal Article
Alternative Title : Cell

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Budkevich, T. V., Author
Giesebrecht, J., Author
Behrmann, E.1, Author
Loerke, J., Author
Ramrath, D. J., Author
Mielke, T., Author
Ismer, J., Author
Hildebrand, P. W., Author
Tung, C. S., Author
Nierhaus, K. H., Author
Sanbonmatsu, K. Y., Author
Spahn, C. M., Author
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1External Organizations, ou_persistent22              

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Free keywords: Amino Acid Sequence Animals Anticodon/metabolism Codon/metabolism Cryoelectron Microscopy Crystallography, X-Ray Humans Mammals/*metabolism Molecular Sequence Data Peptide Chain Elongation, Translational RNA, Transfer/metabolism Rabbits Ribosomes/*chemistry Saccharomyces cerevisiae/metabolism Tetrahymena thermophila/metabolism
 Abstract: The extent to which bacterial ribosomes and the significantly larger eukaryotic ribosomes share the same mechanisms of ribosomal elongation is unknown. Here, we present subnanometer resolution cryoelectron microscopy maps of the mammalian 80S ribosome in the posttranslocational state and in complex with the eukaryotic eEF1AVal-tRNAGMPPNP ternary complex, revealing significant differences in the elongation mechanism between bacteria and mammals. Surprisingly, and in contrast to bacterial ribosomes, a rotation of the small subunit around its long axis and orthogonal to the well-known intersubunit rotation distinguishes the posttranslocational state from the classical pretranslocational state ribosome. We term this motion "subunit rolling." Correspondingly, a mammalian decoding complex visualized in substates before and after codon recognition reveals structural distinctions from the bacterial system. These findings suggest how codon recognition leads to GTPase activation in the mammalian system and demonstrate that in mammalia subunit rolling occurs during tRNA selection.

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 Dates: 2014
 Publication Status: Issued
 Pages: -
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 Rev. Type: -
 Identifiers: Other: 24995983
DOI: 10.1016/j.cell.2014.04.044
ISSN: 1097-4172 (Electronic)
ISSN: 0092-8674 (Linking)
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Title: Cell
  Alternative Title : Cell
Source Genre: Journal
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Pages: - Volume / Issue: 158 (1) Sequence Number: - Start / End Page: 121 - 31 Identifier: -