English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Detecting endogenous SUMO targets in mammalian cells and tissues.

Becker, J., Barysch, S. V., Karaca, S., Dittmer, C., Hsiao, H. H., Diaz, M. B., et al. (2013). Detecting endogenous SUMO targets in mammalian cells and tissues. Nature Structural and Molecular Biology, 20(4), 525-531. doi:10.1038/nsmb.2526.

Item is

Files

show Files
hide Files
:
1740152.pdf (Publisher version), 903KB
Name:
1740152.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
1740152_Supplement_1.pdf (Supplementary material), 3MB
Name:
1740152_Supplement_1.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Becker , J., Author
Barysch, S. V., Author
Karaca, S.1, Author           
Dittmer, C., Author
Hsiao, H. H.1, Author           
Diaz , M. B., Author
Herzig, S., Author
Urlaub, H.1, Author           
Melchior, F., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

Content

show
hide
Free keywords: -
 Abstract: SUMOylation is an essential modification that regulates hundreds of proteins in eukaryotic cells. Owing to its dynamic nature and low steady-state levels, endogenous SUMOylation is challenging to detect. Here, we present a method that allows efficient enrichment and identification of endogenous targets of SUMO1 and the nearly identical SUMO2 and 3 (SUMO 2/3) from vertebrate cells and complex organ tissue. Using monoclonal antibodies for which we mapped the epitope, we enriched SUMOylated proteins by immunoprecipitation and peptide elution. We used this approach in combination with MS to identify SUMOylated proteins, which resulted in the first direct comparison of the endogenous SUMO1- and SUMO2/3-modified proteome in mammalian cells, to our knowledge. This protocol provides an affordable and feasible tool to investigate endogenous SUMOylation in primary cells, tissues and organs, and it will facilitate understanding of SUMO's role in physiology and disease.

Details

show
hide
Language(s): eng - English
 Dates: 2013-03-172013-04
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nsmb.2526
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Structural and Molecular Biology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 20 (4) Sequence Number: - Start / End Page: 525 - 531 Identifier: -