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  Model membrane systems

Keller, H., Worch, R., & Schwille, P. (2013). Model membrane systems. In M. Williams, & T. Daviter (Eds.), Protein-Ligand Interactions. Methods in Molecular Biology, vol 1008 (pp. 417-438). Totowa, NJ.: Humana Press.

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 Creators:
Keller, Heiko, Author
Worch, Remigiusz, Author
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: Model membranes - Artificial lipid membranes - Liposomes - Small unilamellar vesicles - Giant unilamellar vesicles - Supported lipid bilayers - Nanodiscs - Plasma membrane sheets - Receptor–ligand interaction
 Abstract: Model membrane systems can be classified according to their shape and origin. Depending on the shape, model membranes are compatible with a wide variety of methods, ranging from biochemical and biophysical solution assays to microscopy. So far, many applications have been focused on studying the membranes themselves, rather than membrane-associated processes like protein–protein or protein–ligand interactions. However, many model membranes with embedded proteins should be amenable to these studies, too, i.e., be suitable samples for many of the techniques discussed in this book. In this chapter, we discuss how some practical limitations that often restrict the use of model membranes can be overcome. After introducing various membrane structures, an overview is given over alternative ways of their production. Finally, several established protocols, which are suitable for beginners in the field, are described in detail.

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Language(s): eng - English
 Dates: 2013-05-20
 Publication Status: Published in print
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-1-62703-398-5_15
 Degree: -

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Title: Protein-Ligand Interactions. Methods in Molecular Biology, vol 1008
  Subtitle : Methods and Applications
Source Genre: Book
 Creator(s):
Williams, M., Editor
Daviter, T., Editor
Affiliations:
-
Publ. Info: Totowa, NJ. : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 417 - 438 Identifier: ISBN: 978-1-62703-397-8