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  FCS in STED microscopy: Studying the nanoscale of lipid membrane dynamics.

Müller, V., Honigmann, A., Ringemann, C., Medda, R., Schwarzmann, G., & Eggeling, C. (2013). FCS in STED microscopy: Studying the nanoscale of lipid membrane dynamics. In S. Y. Tetin (Ed.), Fluorescence Fluctuation Spectroscopy (FFS), Part B (pp. 1-38). San Diego, Calif.: Elsevier Acad. Pr.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0013-B020-9 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-0027-CF00-A
Genre: Buchkapitel

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 Urheber:
Müller, V.1, Autor           
Honigmann, A.1, Autor           
Ringemann, C.1, Autor           
Medda, R.1, Autor           
Schwarzmann, G., Autor
Eggeling, C.1, Autor           
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Schlagwörter: -
 Zusammenfassung: Details of molecular membrane dynamics in living cells such as lipid protein interactions or the incorporation of molecules into lipid "rafts" are often hidden to the observer because of the limited spatial resolution of conventional far-field optical microscopy. Fortunately, the superior spatial resolution of far-field stimulated-emission-depletion (STED) nanoscopy allows gaining new insights. Applying fluorescence correlation spectroscopy (FCS) in focal spots continuously tuned down to 30 nm in diameter distinguishes free from anomalous molecular diffusion due to transient binding, as for the diffusion of fluorescent phosphoglycero- and sphingolipid analogs in the plasma membrane of living cells. STED-FCS data recorded at different environmental conditions and on different lipid analogs reveal molecular details of the observed nanoscale trapping. Dependencies on the molecular structure of the lipids point to the distinct connectivity of the various lipids to initiate or assist cellular signaling events, but also outline strong differences to the characteristics of liquid-ordered and disordered phase separation in model membranes. STED-FCS is a highly sensitive and exceptional tool to study the membrane organization by introducing a new class of nanoscale biomolecular studies.

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Sprache(n): eng - English
 Datum: 2013
 Publikationsstatus: Erschienen
 Seiten: L, 325, [18]
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/B978-0-12-405539-1.00001-4
 Art des Abschluß: -

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Quelle 1

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Titel: Fluorescence Fluctuation Spectroscopy (FFS), Part B
Genre der Quelle: Buch
 Urheber:
Tetin, S. Y., Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: San Diego, Calif. : Elsevier Acad. Pr.
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: 1 - 38 Identifikator: ISBN: 978-0-12-405539-1

Quelle 2

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Titel: Methods in Enzymology
Genre der Quelle: Reihe
 Urheber:
Abelson, J. N., Herausgeber
Melvin, I. S., Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 519 Artikelnummer: - Start- / Endseite: - Identifikator: -