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  Cloning and functional characterization of the amphibian mesotocin receptor, a member of the oxytocin/vasopressin receptor superfamily

Akhundova, A., Getmanova, E., Gorbulev, V., Carnazzi, E., Eggena, P., & Fahrenholz, F. (1996). Cloning and functional characterization of the amphibian mesotocin receptor, a member of the oxytocin/vasopressin receptor superfamily. European Journal of Biochemistry, 237(3), 759-767. doi:10.1111/j.1432-1033.1996.0759p.x.

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Akhundova, Aida1, Autor           
Getmanova, Elena1, Autor           
Gorbulev, Valentin1, Autor           
Carnazzi, Eric1, Autor           
Eggena, Patrick2, Autor
Fahrenholz, Falk1, Autor           
Affiliations:
1Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              
2The Mount Sinai Medical Center, New York, USA, ou_persistent22              

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Schlagwörter: mesotocin receptor; molecular evolution; terrestrial amphibians; water transport; hormone‐binding site
 Zusammenfassung: Mesotocin is the oxytocin-like hormone found in most terrestrial vertebrates from lungfishes to marsupials, which includes all non-mammalian tetrapods (amphibians, reptiles, and birds). It has the largest distribution in vertebrates after vasotocin found in all non-mammalian vertebrates and isotocin identified in bony fishes. In this study, we report the cloning and functional characterization of the cDNA for the mesotocin receptor (MTR) from the urinary bladder of the toad Bufo marinus. The cloned cDNA encodes a polypeptide of 389 amino acids that shows the greatest similarity to the teleost fish isotocin receptor and to mammalian oxytocin receptors with mutations in extracellular loops which are involved in ligand binding. When expressed in COSM6 cells, MTR exhibits the following relative order of ligand affinity: mesotocin > vasotocin = oxytocin > vasopressin > hydrin 1, isotocin, hydrin 2. Injection of MTR cRNA into Xenopus laevis oocytes induces membrane chloride currents in response to mesotocin, which indicates the coupling of the mesotocin receptor to the inositol phosphate/calcium pathway. This response is inhibited by an oxytocin antagonist, but not by a vasopressin antagonist specific for V2 vasopressin receptors. MTR mRNA is not only found in toad urinary bladder, but also in kidney, muscle, and brain tissue of the toad as revealed by northern blot analysis and reverse-transcriptase PCR. The results suggest a variety of function for mesotocin and its receptor including, in particular, an involvement in the regulation of water and salt transport.

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Sprache(n): eng - English
 Datum: 1995-12-132004-08-311996-05-01
 Publikationsstatus: Erschienen
 Seiten: 9
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1111/j.1432-1033.1996.0759p.x
PMID: 8647123
 Art des Abschluß: -

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Titel: European Journal of Biochemistry
Genre der Quelle: Zeitschrift
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Affiliations:
Ort, Verlag, Ausgabe: Berlin : Published by Springer-Verlag on behalf of the Federation of European Biochemical Societies
Seiten: - Band / Heft: 237 (3) Artikelnummer: - Start- / Endseite: 759 - 767 Identifikator: ISSN: 0014-2956
CoNE: https://pure.mpg.de/cone/journals/resource/111097776606040