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  Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors

Selhuber-Unkel, C., Erdmann, T., López-García, M., Kessler, H., Schwarz, U. S., & Spatz, J. P. (2010). Cell adhesion strength is controlled by intermolecular spacing of adhesion receptors. Biophysical Journal, 98(4), 543-551. doi:10.1016/j.bpj.2009.11.001.

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BiophysJ_98_2010_543.pdf (Any fulltext), 661KB
 
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Selhuber-Unkel, Christine1, Author           
Erdmann, T., Author
López-García, M., Author
Kessler, H., Author
Schwarz, U. S., Author
Spatz, Joachim P.1, 2, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: Spatial patterning of biochemical cues on the micro- and nanometer scale controls numerous cellular processes such as spreading, adhesion, migration, and proliferation. Using force microscopy we show that the lateral spacing of individual integrin receptor-ligand bonds determines the strength of cell adhesion. For spacings > or = 90 nm, focal contact formation was inhibited and the detachment forces as well as the stiffness of the cell body were significantly decreased compared to spacings < or = 50 nm. Analyzing cell detachment at the subcellular level revealed that rupture forces of focal contacts increase with loading rate as predicted by a theoretical model for adhesion clusters. Furthermore, we show that the weak link between the intra- and extracellular space is at the intracellular side of a focal contact. Our results show that cells can amplify small differences in adhesive cues to large differences in cell adhesion strength.

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Language(s): eng - English
 Dates: 2009-07-022009-11-022010-02-17
 Publication Status: Issued
 Pages: 9
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 Rev. Type: Peer
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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 98 (4) Sequence Number: - Start / End Page: 543 - 551 Identifier: Other: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117