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  Two-color 810 nm STED nanoscopy of living cells with endogenous SNAP-tagged fusion proteins.

Butkevich, A., Ta, H., Ratz, M., Stoldt, S., Jakobs, S., Belov, V. N., et al. (2018). Two-color 810 nm STED nanoscopy of living cells with endogenous SNAP-tagged fusion proteins. ACS Chemical Biology, 13(2), 475-480. doi:10.1021/acschembio.7b00616.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002D-FCD8-A Version Permalink: https://hdl.handle.net/21.11116/0000-0003-476C-5
Genre: Journal Article

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 Creators:
Butkevich, A.1, Author           
Ta, H.1, Author           
Ratz, M.2, Author           
Stoldt, S.2, Author           
Jakobs, S.2, Author           
Belov, V. N.1, Author           
Hell, S. W.1, Author           
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

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 Abstract: A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell superresolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP-tag, together with a non-covalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.

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Language(s): eng - English
 Dates: 2017-09-212018-02-16
 Publication Status: Issued
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 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acschembio.7b00616
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Title: ACS Chemical Biology
Source Genre: Journal
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Pages: - Volume / Issue: 13 (2) Sequence Number: - Start / End Page: 475 - 480 Identifier: -