Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  Separate pathways contribute to the herbivore-induced formation of 2-phenylethanol in poplar

Günther, J., Lackus, N., Schmidt, A., Huber, M., Stödtler, H.-J., Reichelt, M., et al. (2019). Separate pathways contribute to the herbivore-induced formation of 2-phenylethanol in poplar. Plant Physiology, 180(2), 767-782. doi:10.1104/pp.19.00059.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
GER545.pdf (Verlagsversion), 2MB
 
Datei-Permalink:
-
Name:
GER545.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-
:
GER545s1.pdf (Ergänzendes Material), 2MB
 
Datei-Permalink:
-
Name:
GER545s1.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Chemical Ecology, MJCO; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
https://doi.org/10.1104/pp.19.00059 (Verlagsversion)
Beschreibung:
OA
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Günther, Jan1, 2, Autor           
Lackus, Nathalie1, 2, Autor           
Schmidt, Axel1, Autor           
Huber, Meret1, Autor           
Stödtler, Heike-Jana1, Autor           
Reichelt, Michael1, Autor           
Gershenzon, Jonathan1, Autor           
Köllner, Tobias G.1, Autor           
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Upon herbivory, the tree species western balsam poplar (Populus trichocarpa) produces a variety of phenylalanine-derived metabolites, including 2-phenylethylamine, 2-phenylethanol, and 2-phenylethyl-β-D-glucopyranoside. To investigate the formation of these potential defense compounds, we functionally characterized aromatic L-amino acid decarboxylases (AADCs) and aromatic aldehyde synthases (AASs), which play important roles in the biosynthesis of specialized aromatic metabolites in other plants. Heterologous expression in Escherichia coli and Nicotiana benthamiana showed that all five AADC/AAS genes identified in the P. trichocarpa genome encode active enzymes. However, only two genes, PtAADC1 and PtAAS1, were significantly upregulated after leaf herbivory. Despite a sequence similarity of about 96%, PtAADC1 and PtAAS1 showed different enzymatic functions and converted phenylalanine into 2-phenylethylamine and 2-phenylacetaldehyde, respectively. The activities of both enzymes were interconvertible by switching a single amino acid residue in their active sites. A survey of putative AADC/AAS gene pairs in the genomes of other plants suggests an independent evolution of this function-determining residue in different plant families. RNAi-mediated downregulation of AADC1 in grey poplar (P. × canescens) resulted in decreased accumulation of 2-phenylethylamine and 2-phenylethyl-β-D-glucopyranoside, while the emission of 2-phenylethanol was not influenced. To investigate the last step of 2-phenylethanol formation, we identified and characterized two P. trichocarpa short-chain dehydrogenases, PtPAR1 and PtPAR2, which were able to reduce 2-phenylacetaldehyde to 2-phenylethanol in vitro. In summary, 2-phenylethanol and its glucoside may be formed in multiple ways in poplar. Our data indicate that PtAADC1 controls the herbivore-induced formation of 2-phenylethylamine and 2-phenylethyl-β-D-glucopyranoside in planta, while PtAAS1 likely contributes to the herbivore-induced emission of 2-phenylethanol.

Details

einblenden:
ausblenden:
Sprache(n):
 Datum: 2019-03-072019-06
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: Anderer: GER545
DOI: 10.1104/pp.19.00059
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Plant Physiology
  Andere : Plant Physiol.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Bethesda, Md. : American Society of Plant Biologists
Seiten: - Band / Heft: 180 (2) Artikelnummer: - Start- / Endseite: 767 - 782 Identifikator: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438