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  Functional expression of GABAA chloride channels and benzodiazepine binding sites in baculovirus infected insect cells

Carter, D. B., Thomsen, D. R., Im, W. B., Lennon, D. J., Ngo, D. M., Gale, W., et al. (1992). Functional expression of GABAA chloride channels and benzodiazepine binding sites in baculovirus infected insect cells. Nature Biotechnology, 10(6), 679-681. doi:10.1038/nbt0692-679.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0000-60CF-B Version Permalink: https://hdl.handle.net/21.11116/0000-0000-60D0-8
Genre: Journal Article

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NatBiotechnol_10_1992_679.pdf (Any fulltext), 419KB
 
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https://www.nature.com/articles/nbt0692-679.pdf (Any fulltext)
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 Creators:
Carter, D. B., Author
Thomsen, D. R., Author
Im, W. B., Author
Lennon, D. J., Author
Ngo, D. M., Author
Gale, W., Author
Im, H. K., Author
Seeburg, Peter H.1, Author           
Smith, M. W., Author
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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 Abstract: We have employed the baculovirus expression system for the production of insect cell membranes having GABA/benzodiazepine binding sites. Three recombinant baculoviruses each harboring a different GABAA receptor cDNA were introduced into insect cells by simultaneous infection. Infected cells expressed GABA responsive Cl- channels and benzodiazepine binding sites with the same pharmacological specificity as animal cells expressing these receptor subunits.

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Language(s): eng - English
 Dates: 1992-01-151992-04-071992-06-011992-06-01
 Publication Status: Issued
 Pages: 3
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nbt0692-679
URI: https://www.ncbi.nlm.nih.gov/pubmed/1369398
URI: https://www.ncbi.nlm.nih.gov/m/pubmed/1369398/
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Title: Nature Biotechnology
Source Genre: Journal
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Publ. Info: New York : Gale Group Inc.
Pages: - Volume / Issue: 10 (6) Sequence Number: - Start / End Page: 679 - 681 Identifier: ISSN: 1087-0156
CoNE: https://pure.mpg.de/cone/journals/resource/954926980065