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Free keywords:
NANOSCALE SHAPES; BAR DOMAINS; FOLDING DNA; PROTEIN; NANOSTRUCTURES;
MECHANISMS; CURVATURE; VIRUSdiffusion; lipid membranes; membrane deformation; oligomerization;
origami DNA structures;
Abstract:
We report a synthetic biology-inspired approach for the engineering of amphipathic DNA origami structures as membrane-scaffolding tools. The structures have a flat membrane-binding interface decorated with cholesterol-derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free-standing lipid membranes, mimicking the biological activity of coat-forming proteins, for example, from the I-/F-BAR family.
