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  Engineering a reversible fluorescent probe for real-time live-cell imaging and quantification of mitochondrial ATP

Ren, T.-B., Wen, S., Wang, L., Lu, P., Xiong, B., Yuan, L., et al. (2020). Engineering a reversible fluorescent probe for real-time live-cell imaging and quantification of mitochondrial ATP. Analytical Chemistry, 92(6), 4681-4688. doi:10.1021/acs.analchem.0c00506.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0005-C01B-4 Version Permalink: https://hdl.handle.net/21.11116/0000-0006-09E0-3
Genre: Journal Article

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 Creators:
Ren, Tian-Bing, Author
Wen, Siyu, Author
Wang, Lu1, Author           
Lu, Peng, Author
Xiong, Bin, Author
Yuan, Lin, Author
Zhang, Xiao-Bing, Author
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: Real-time imaging and quantification of adenosine triphosphate (ATP) dynamics in living cells are important for understanding relationship between energy metabolism and cell functions. However, few synthetic fluorescent probes have been reported to tackle this challenge due to lack of accurate fluorescence readout and suitable response concentration. In this work, we developed a ratiometric fluorescent probe (Rh6G-ACFPN) for quantitatively monitoring the fluctuation of mitochondrial ATP in living cells. Rh6G-ACFPN selectively and reversibly responds to ATP with an ideal dissociation constant (Kd) of 4.72 mM (3–10 mM: the range of mitochondrial ATP concentrations). Live-cell imaging allows us to directly monitor the dynamic changes of mitochondrial ATP in high temporal resolution. Moreover, for the first time, mitochondrial ATP in normal and cancer cells lines was successfully quantified and discriminated. These results demonstrate the versatility of Rh6G-ACFPN as a useful imaging tool to elucidate the function of mitochondrial ATP in living cells.

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Language(s): eng - English
 Dates: 2020-02-042020-02-262020-02-262020-03-17
 Publication Status: Issued
 Pages: 21
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acs.analchem.0c00506
URI: https://www.ncbi.nlm.nih.gov/pubmed/32098468
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Title: Analytical Chemistry
  Abbreviation : Anal. Chem.
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 92 (6) Sequence Number: - Start / End Page: 4681 - 4688 Identifier: ISSN: 0003-2700
CoNE: https://pure.mpg.de/cone/journals/resource/111032812862552