ausblenden:
Schlagwörter:
glutamic acid decarboxylase, interneurons, digoxigenin, cRNA probes, hippocampus
Zusammenfassung:
The physiological properties of a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type glutamate
receptor (GIuR) channels are determined by their subunit composition. In particular, the expression of the GIuRB
subunit determines the divalent ion selectivity of the channel. We studied the distribution of GIuR-B mRNA and
protein in adult rat hippocampal GABAergic neurons combining non-radioactive in sifu hybridization with
immunocytochemistry. The majority of GABAergic hippocampal neurons were GIuR-B mRNA-positive, but overall
the levels of GIuR-B transcript were lower than in pyramidal and granule cells, which showed the highest
hybridization signal. The different GIuR-B mRNA expression levels in GABAergic versus principal neurons were
also observed at the protein level. There was a paucity of GIuR-B/C immunoreactivity in the vast majority of
somata of the GABAergic neurons studied, which contrasted with the strong expression of GIuR-B/C proteins in
the hippocampal principal neurons. Our results demonstrate the general low expression of the GIuR-B subunit
mRNA and GIuR-B/C proteins in GABAergic hippocampal neurons. Considering the dominant role of the GIuR-B
subunit in determining the divalent ion permeability of the receptor, it is likely
