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  Extracellular Vesicle Isolation and Characterization from Periprosthetic Joint Synovial Fluid in Revision Total Joint Arthroplasty

Ruewald, J. M., Randau, T. M., Hilgers, C., Masson, W., Irsen, S., Eymael, R. L., et al. (2020). Extracellular Vesicle Isolation and Characterization from Periprosthetic Joint Synovial Fluid in Revision Total Joint Arthroplasty. Journal of Clinical Medicine, 9(2): 516. doi:10.3390/jcm9020516.

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jcm-09-00516-v2.pdf (Publisher version), 2MB
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https://www.mdpi.com/2077-0383/9/2/516 (Publisher version)
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 Creators:
Ruewald, Julian M.1, Author
Randau, Thomas M.1, Author
Hilgers, Caecilia1, Author
Masson, Werner1, Author
Irsen, Stephan2, Author              
Eymael, Robin L.1, Author
Kohlhof, Hendrik1, Author
Gravius, Sascha1, Author
Burger, Christof1, Author
Wirtz, Dieter C.1, Author
Schildberg, Frank A.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Electron Microscopy and Analytics, Center of Advanced European Studies and Research (caesar), Max Planck Society, ou_2173680              

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 Abstract: Extracellular vesicles (EVs) comprise an as yet insufficiently investigated intercellular communication pathway in the field of revision total joint arthroplasty (RTJA). This study examined whether periprosthetic joint synovial fluid contains EVs, developed a protocol for their isolation and characterized them with respect to quantity, size, surface markers as well as documented their differences between aseptic implant failure (AIF) and periprosthetic joint infection (PJI). EV isolation was accomplished using ultracentrifugation, electron microscopy (EM) and nanoparticle tracking analysis evaluated EV presence as well as particle size and quantity. EV surface markers were studied by a bead-based multiplex analysis. Using our protocol, EM confirmed the presence of EVs in periprosthetic joint synovial fluid. Higher EV particle concentrations and decreased particle sizes were apparent for PJI. Multiplex analysis confirmed EV-typical surface epitopes and revealed upregulated CD44 and HLA-DR/DP/DQ for AIF, as well as increased CD40 and CD105. Our protocol achieved isolation of EVs from periprosthetic joint synovial fluid, confirmed by EM and multiplex analysis. Characterization was documented with respect to size, concentration and epitope surface signature. Our results indicate various differences between PJI and AIF EVs. This pilot study enables new research approaches and rising diagnostic opportunities in the field of RTJA.

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Language(s): eng - English
 Dates: 2020-02-14
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000518823000228
DOI: 10.3390/jcm9020516
 Degree: -

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Title: Journal of Clinical Medicine
Source Genre: Journal
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Publ. Info: Basel : MDPI
Pages: - Volume / Issue: 9 (2) Sequence Number: 516 Start / End Page: - Identifier: ISSN: 2077-0383
CoNE: https://pure.mpg.de/cone/journals/resource/2077-0383