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  Electrophysiological Characterization of Membrane Transport Proteins

Grewer, C., Gameiro, A., Mager, T., & Fendler, K. (2013). Electrophysiological Characterization of Membrane Transport Proteins. Annual Review of Biophysics, 42, 95-120.

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 Urheber:
Grewer, Christof1, Autor           
Gameiro, Armanda, Autor
Mager, Thomas1, Autor           
Fendler, Klaus1, Autor           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

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Schlagwörter: active transport; caged substrates; rapid kinetics; charge displacement; electrogenic step; conformational transition
 Zusammenfassung: Active transport in biological membranes has been traditionally studied using a variety of biochemical and biophysical techniques, including electrophysiology. This review focuses on aspects of electrophysiological methods that make them particularly suited for the investigation of transporter function. Two major approaches to electrical recording of transporter activity are discussed: (a) artificial planar lipid membranes, such as the black lipid membrane and solid supported membrane, which are useful for studies on bacterial transporters and transporters of intracellular compartments, and (b) patch clamp and voltage clamp techniques, which investigate transporters in native cellular membranes. The analytical power of these methods is highlighted by several examples of mechanistic studies of specific membrane proteins, including cytochrome c oxidase, NhaA Na+/H+ exchanger, ClC-7 H+/Cl exchanger, glutamate transporters, and neutral amino acid transporters. These examples reveal the wealth of mechanistic information that can be obtained when electrophysiological methods are used in combination with rapid perturbation approaches.

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Sprache(n): eng - English
 Datum: 2013-05
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Interne Begutachtung
 Identifikatoren: eDoc: 670321
 Art des Abschluß: -

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Titel: Annual Review of Biophysics
  Alternativer Titel : Annu Rev Biophys
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 42 Artikelnummer: - Start- / Endseite: 95 - 120 Identifikator: -