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  Liquid Phase Separation Controlled by pH

Adame-Arana, O., Weber, C. A., Zaburdaev, V., Prost, J., & Julicher, F. (2020). Liquid Phase Separation Controlled by pH. Biophysical Journal, 119(8), 1590-1605. doi:10.1016/j.bpj.2020.07.044.

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 Urheber:
Adame-Arana, Omar1, Autor
Weber, Christoph A.1, Autor
Zaburdaev, Vasily2, 3, Autor           
Prost, Jacques1, Autor
Julicher, Frank1, Autor
Affiliations:
1external, ou_persistent22              
2Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164414              
3University of Erlangen-Nürnberg, ou_persistent22              

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 Zusammenfassung: We present a minimal model to study the effects of pH on liquid phase separation of macromolecules. Our model describes a mixture composed of water and macromolecules that exist in three different charge states and have a tendency to phase separate. This phase separation is affected by pH via a set of chemical reactions describing protonation and deprotonation of macromolecules, as well as self-ionization of water. We consider the simple case in which interactions are captured by Flory-Huggins interaction parameters corresponding to Debye screening lengths shorter than a nanometer, which is relevant to proteins inside biological cells under physiological conditions. We identify the conjugate thermodynamic variables at chemical equilibrium and discuss the effective free energy at fixed pH. First, we study phase diagrams as a function of macromolecule concentration and temperature at the isoelectric point of the macromolecules. We find a rich variety of phase diagram topologies, including multiple critical points, triple points, and first-order transition points. Second, we change the pH relative to the isoelectric point of the macromolecules and study how phase diagrams depend on pH. We find that these phase diagrams as a function of pH strongly depend on whether oppositely charged macromolecules or neutral macromolecules have a stronger tendency to phase separate. One key finding is that we predict the existence of a reentrant behavior as a function of pH. In addition, our model predicts that the region of phase separation is typically broader at the isoelectric point. This model could account for both in vitro phase separation of proteins as a function of pH and protein phase separation in yeast cells for pH values close to the isoelectric point of many cytosolic proteins.

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Sprache(n): eng - English
 Datum: 2020-10-20
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000579362500015
DOI: 10.1016/j.bpj.2020.07.044
 Art des Abschluß: -

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Titel: Biophysical Journal
Genre der Quelle: Zeitschrift
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Affiliations:
Ort, Verlag, Ausgabe: Cell Press
Seiten: - Band / Heft: 119 (8) Artikelnummer: - Start- / Endseite: 1590 - 1605 Identifikator: ISSN: 0006-3495