hide
Free keywords:
FAMATA DEBARYOMYCES-HANSENII; CANDIDA-FAMATA; D-FABRYI; TRANSFORMATION;
CONSTRUCTION; TROPICALIS; GENECandida famata; riboflavin; SEF1; SFU1; VMA1; yeast;
Abstract:
Riboflavin or vitamin B(2)is an essential dietary component for humans and animals that is the precursor of flavin coenzymes flavin mononucleotide and flavin adenine dinucleotide involved in numerous enzymatic reactions. The flavinogenic yeastCandida famataoverproduces riboflavin under iron starvation; however, regulation of this process is poorly understood. Regulatory geneSEF1encoding transcription activator has been identified. Its deletion blocks yeast ability to overproduce riboflavin under iron starvation. It was shown here that theSEF1promoters from other flavinogenic (Candida albicans) and non-flavinogenic (Candida tropicalis) yeasts fused with the open reading frame (ORF) ofSEF1gene fromC. famataare able to restore riboflavin oversynthesis insef1 Delta mutants. It is known that in the pathogenic flavinogenic yeastC. albicans, Sfu1 (GATA-type transcription factor) repressesSEF1.Here, we found that deletion ofSFU1gene in wild-typeC. famataleads to riboflavin oversynthesis. Moreover, it was shown that disruption ofVMA1gene (coding for vacuolar ATPase subunit A) also results in riboflavin oversynthesis inC. famata.
