ausblenden:
Schlagwörter:
Picosecond Infrared laser (PIRL), desorption impulsive vibrational excitation (DIVE), Top-down proteomic
Zusammenfassung:
Introduction: A novel method for ultrafast and soft cold vaporization of tissue via desorption by impulsiv
e vibrational excitation (DIVE) using a
picosecond
-
infrared laser (PIRL) has been developed by Dwayne Miller and his group [1]. By irradiating tissue with PIRL proteins
are transferred from the tissue into the gas phase within at aerosol plume without chan
ging the chemical formula of protein. The
chemical composition of the protein is maintained during tissue ablation via PIRL. In this study, the efficiency of tissue
homogenization performed with PIRL and with classical homogenization was investigated using
two
-
dimensional
electrophoresis (2DE) followed by mass spectrometric analysis (MS) of the tryptic peptides of the separated proteins from the
2DE
gel.
