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  A proteomic workflow for characterization of human skin biopsies by using pico-second infrared laser (PIRL)

Kiani, P., Eggert, D., Bussmann, T., Reuter, J. H., Uschold, S., Hansen, N.-O., et al. (2016). A proteomic workflow for characterization of human skin biopsies by using pico-second infrared laser (PIRL). Poster presented at 49. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie (DGMS), Hamburg.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0001-DE42-B Version Permalink: https://hdl.handle.net/21.11116/0000-0001-DE43-A
Genre: Poster

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https://www.dgms2016.uni-hamburg.de/program/dgms2016-abstracts-poster.pdf (Any fulltext)
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 Creators:
Kiani, P.1, Author
Eggert, D.2, Author
Bussmann, T.3, Author
Reuter, J. H.3, Author
Uschold, S.4, Author           
Hansen, N.-O.4, Author           
Miller, R. J. D.4, Author           
Schlüter, H.1, Author
Affiliations:
1University Medical Center Hamburg-Eppendorf, Institute for Clinical Chemistry, Mass Spectrometric Proteomics Group, ou_persistent22              
2Heinrich Pette Institute, Leibniz Institute for Experimental Virology, ou_persistent22              
3k.A., ou_persistent22              
4Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              

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Free keywords: Proteomics, DIVE, PIRL, tissue ablation, tandem mass spectrometry
 Abstract: Introduction: Human skin is the largest organ of integumentary system that protect the body from e xternal environment and prevent excessive fluid loss [1]. Scientists are closer than ever to understanding the proteomes of skin [2]. However, the protein compositions in each type of cell layers, especially in epidermis, are not accurately defined. For fu rther characterization of protein composition in layers of human skin we have undertaken a proteomic approach by using an innovative, “one - step” extraction and homogenization method, called DIVE (desorption by impulsive excitation of intramolecular vibrati onal states of water molecules in the cell), induced by irradiating tissue with a pico - second infrared laser (PIRL) [3]. The aim of this study was to test if with DIVE epidermis and dermis can be ablated separately.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Not specified
 Pages: 1
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 Rev. Type: No review
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Title: 49. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie (DGMS)
Place of Event: Hamburg
Start-/End Date: 2016-02-28 - 2016-03-02

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