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Free keywords:
HAIR FOLLICLE MORPHOGENESIS; ADULT-MOUSE EPIDERMIS; KINDLER-SYNDROME;
FOCAL ADHESION; COMPREHENSIVE GUIDE; TUMOR-INDUCTION; SKIN; ACTIVATION;
EXPRESSION; CATENIN
Abstract:
Kindlin-1 is an integrin tail binding protein that controls integrin activation. Mutations in the FERMT-1 gene, which encodes for Kindlin-1, lead to Kindler syndrome in man, which is characterized by skin blistering, premature skin aging and skin cancer of unknown etiology. Here we show that loss of Kindlin-1 in mouse keratinocytes recapitulates Kindler syndrome and also produces enlarged and hyperactive stem cell compartments, which lead to hyperthickened epidermis, ectopic hair follicle development and increased skin tumor susceptibility. Mechanistically, Kindlin-1 controls keratinocyte adhesion through beta 1-class integrins and proliferation and differentiation of cutaneous epithelial stem cells by promoting alpha(nu)beta(6) integrin-mediated transforming growth factor-beta (TGF-beta) activation and inhibiting Wnt-beta-catenin signaling through integrin-independent regulation of Wnt ligand expression. Our findings assign Kindlin-1 the previously unknown and essential task of controlling cutaneous epithelial stem cell homeostasis by balancing TGF-beta-mediated growth-inhibitory signals and Wnt-beta-catenin-mediated growth-promoting signals.
