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Free keywords:
Amino Acid Sequence; Base Sequence; Blotting, Western; Cloning, Molecular; *Cysteine Endopeptidases/ge [Genetics]; Cysteine Endopeptidases/ip [Isolation & Purification]; Cysteine Endopeptidases/me [Metabolism]; DNA, Bacterial; Electrophoresis, Gel, Two-Dimensional; Escherichia coli; Microscopy, Electron; Molecular Sequence Data; *Multienzyme Complexes/ge [Genetics]; Multienzyme Complexes/ip [Isolation & Purification]; Multienzyme Complexes/me [Metabolism]; Recombinant Proteins/ge [Genetics]; Recombinant Proteins/ip [Isolation & Purification]; Recombinant Proteins/me [Metabolism]; Thermoplasma/en [Enzymology]; *Thermoplasma/ge [Genetics]
Abstract:
The two genes encoding the constituent subunits of the Thermoplasma acidophilum proteasome were expressed in Escherichia coli yielding fully assembled molecules as shown by electron microscopy. The recombinant proteasomes were purified to homogeneity and were shown to have proteolytic activity indistinguishable from proteasomes isolated from T. acidophilum.
