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  Persistent inhibition of pore-based cell migration by sub-toxic doses of miuraenamide, an actin filament stabilizer

Moser, C., Ruediger, D., Foerster, F., von Blume, J., Yu, P., Kuster, B., et al. (2017). Persistent inhibition of pore-based cell migration by sub-toxic doses of miuraenamide, an actin filament stabilizer. Scientific Reports, 7: 16407. doi:10.1038/s41598-017-16759-7.

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 Urheber:
Moser, Christina1, Autor
Ruediger, Daniel1, Autor
Foerster, Florian1, Autor
von Blume, Julia2, Autor           
Yu, Peng1, Autor
Kuster, Bernhard1, Autor
Kazmaier, Uli1, Autor
Vollmar, Angelika M.1, Autor
Zahler, Stefan1, Autor
Affiliations:
1external, ou_persistent22              
2von Blume, Julia / Molecular Basis of Protein Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565173              

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Schlagwörter: SERUM RESPONSE FACTOR; NATURAL-PRODUCTS; F-ACTIN; MRTF-A; CYTOSKELETON; BINDING; CANCER; IDENTIFICATION; CHONDRAMIDES; CONFINEMENTScience & Technology - Other Topics;
 Zusammenfassung: Opposed to tubulin-binding agents, actin-binding small molecules have not yet become part of clinical tumor treatment, most likely due to the fear of general cytotoxicity. Addressing this problem, we investigated the long-term efficacy of sub-toxic doses of miuraenamide, an actin filament stabilizing natural compound, on tumor cell (SKOV3) migration. No cytotoxic effects or persistent morphological changes occurred at a concentration of miuraenamide of 20 nM. After 72 h treatment with this concentration, nuclear stiffness was increased, causing reduced migration through pores in a Boyden chamber, while cell migration and chemotaxis per se were unaltered. A concomitant time-resolved proteomic approach showed down regulation of a protein cluster after 56 h treatment. This cluster correlated best with the Wnt signaling pathway. A further analysis of the actin associated MRTF/SRF signaling showed a surprising reduction of SRF-regulated proteins. In contrast to acute effects of actin-binding compounds on actin at high concentrations, long-term low-dose treatment elicits much more subtle but still functionally relevant changes beyond simple destruction of the cytoskeleton. These range from biophysical parameters to regulation of protein expression, and may help to better understand the complex biology of actin, as well as to initiate alternative regimes for the testing of actin-targeting drugs.

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Sprache(n): eng - English
 Datum: 2017
 Publikationsstatus: Online veröffentlicht
 Seiten: 11
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000416308200015
DOI: 10.1038/s41598-017-16759-7
 Art des Abschluß: -

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Titel: Scientific Reports
  Kurztitel : Sci. Rep.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: London, UK : Nature Publishing Group
Seiten: - Band / Heft: 7 Artikelnummer: 16407 Start- / Endseite: - Identifikator: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322