Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
 
 
DownloadE-Mail
 DetailsÜbersicht
  Measurement of bacterivory by heterotrophic nanoflagellates using immunofluorescence labelling of ingested cells

Christoffersen, K., Nybroe, O., Jürgens, K., & Hansen, M. (1997). Measurement of bacterivory by heterotrophic nanoflagellates using immunofluorescence labelling of ingested cells. Aquatic Microbial Ecology, 13(1), 127-134.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-E233-C Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-E234-A
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Christoffersen, K., et al., 1997, S-37286.pdf (Verlagsversion), 683KB
 
Datei-Permalink:
-
Name:
Christoffersen, K., et al., 1997, S-37286.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Evolutionary Biology, MPLM; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Christoffersen, Kirsten, Autor
Nybroe, Ole, Autor
Jürgens, Klaus1, Autor           
Hansen, Michael, Autor
Affiliations:
1Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_976547              

Inhalt

einblenden:
ausblenden:
Schlagwörter: protists; grazing; bacteria; immunofluorescence labelling
 Zusammenfassung: A method based on fluorescent-antibody labelled bacteria (FALB) was developed to demonstrate the presence of ingested bacteria in the food vacuoles of heterotrophic nanoflagellates. Individual bacterial cells are identified in the food vacuoles by immunofluorescence labelling using strain-specific antibodies after uptake of the live cells. The procedure includes permeabilization of fixed flagellate cells prior to the immunoreaction. The uptake of Pseudomonas fluorescens (strain ON2 and Ag1) and P. putida (strain MM1) by Spumella sp., Bodo saltans and 2 unidentified heterotrophic nanoflagellate species was tested. The ingested bacteria were visualized in the food vacuoles by epifluorescence and laser confocal scanning microscopy. The ability of the polyclonal antibodies to recognize the target bacteria was good and very few cross-reactions with other bacteria or other organic compounds were observed. A linear uptake of live bacteria was recorded during the first 15 min of the feeding period and immunolabelled remains of the bacteria were visible inside and around the food vacuoles after extended feeding periods. Ingestion rates measured by the FALB method were significantly higher than those measured by FLB which confirms conclusions from several recent studies using vial stains to detect grazing on bacteria. It was concluded that the FALB method is useful and suitable to examine feeding of protists on specific bacteria as well as differential uptake of bacteria within a mixture. The FALB method does not involve any manipulations of the bacterial cells before the feeding experiment and thus measures realistic ingestion rates

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 1997-07-24
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 206507
Anderer: 1657/S 37286
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Aquatic Microbial Ecology
  Alternativer Titel : Aquat Microb Ecol
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 13 (1) Artikelnummer: - Start- / Endseite: 127 - 134 Identifikator: ISSN: 0948-3055