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Free keywords:
preparative chromatography; injection solvent; column overloading; solubility limits
Abstract:
The separation of compounds possessing low solubility in the mobile phase could be improved by applying stronger solvents for dissolving the feed. A model system has been investigated with ethanol-water as the mobile phase, Chirobiotic T as the stationary phase and DL-threonine as the sample dissolved in pure water. The adsorption isotherms of DLthreonine were determined as a function of the water content by means of a peak fitting method. Optimal injection conditions of elution chromatography were determined by process simulation. Finally, the optima obtained were verified with experimental data.
Copyright © 2013 Elsevier B.V. [accessed 2013 November 26th]