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  Asp85 is the only internal aspartic acid that gets protonated in the M intermediate and the purple-to-blue transition of bacteriorhodopsin. A solid-state 13C CP-MAS NMR investigation

Metz, G., Siebert, F., & Engelhard, M. (1992). Asp85 is the only internal aspartic acid that gets protonated in the M intermediate and the purple-to-blue transition of bacteriorhodopsin. A solid-state 13C CP-MAS NMR investigation. FEBS Letters, 303(2-3), 237-4. doi:10.1016/0014-5793(92)80528-O.

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 Creators:
Metz, Günther1, Author
Siebert, Friedrich1, 2, Author              
Engelhard, Martin3, Author
Affiliations:
1Institut für Biophysik und Strahlenbiologie der Universität, Freiburg, Germany., ou_persistent22              
2Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society, ou_3273415              
3Max-Planck-Institut für Ernährungsphysiologie, 4600 Dortmund, Germany, ou_persistent22              

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Free keywords: Blue bacteriorhodopsin; Solid-state nuclear magnetic resonance (NMR); Frotonation change; Photocycle
 Abstract: High-resolution solid-state 13C NMR spectra of the ground state and M intermediate of the bacteriorhodopsin mutant D96N with the isotope label at [4-13C]Asp and [11-13C]Trp were recorded. The NMR spectra show that Asp85 is protonated in the M intermediate. The environment of Asp85 is quite hydrophobic. On the other hand, Asp212 remains deprotonated and a slight shift to lower field indicates a more hydrophilic environment. Asp85 also protonates in the purple-to-blue transition of bacteriorhodopsin in the deionized membrane, where it experiences a similar environment to M. The shift of Trp resonances in M reflect a conformational change of the protein in forming the M intermediate.

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Language(s): eng - English
 Dates: 1992-04-071992-03-092002-01-161992-06-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0014-5793(92)80528-O
PMID: 1318849
 Degree: -

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Title: FEBS Letters
  Other : FEBS Lett.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 303 (2-3) Sequence Number: - Start / End Page: 237-4 Identifier: ISSN: 0014-5793
CoNE: https://pure.mpg.de/cone/journals/resource/954925399501