English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Single molecule fluorescence spectroscopy of mutants of the Discosoma red fluorescent protein DsRed

Blum, C., Subramaniam, V., Schleifenbaum, F., Stracke, F., Angres, B., Terskikh, A., et al. (2002). Single molecule fluorescence spectroscopy of mutants of the Discosoma red fluorescent protein DsRed. Chemical Physics Letters, 362(5-6), 355-361. Retrieved from http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TFN-46FXSPS-2-H&_cdi=5231&_user=38661&_pii=S000926140201014X&_origin=search&_coverDate=08%2F26%2F2002&_sk=996379994&view=c&wchp=dGLbVzb-zSkzS&md5=e3c12577fbd8643f1fd9cdb548148dc0&ie=/sdarticle.pdf.

Item is

Files

show Files
hide Files
:
16646.pdf (Publisher version), 0B
Name:
16646.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Blum, C., Author
Subramaniam, V.1, Author           
Schleifenbaum, F., Author
Stracke, F., Author
Angres, B., Author
Terskikh, A., Author
Meixner, A. J., Author
Affiliations:
1Department of Molecular Biology, MPI for biophysical chemistry, Max Planck Society, ou_578628              

Content

show
hide
Free keywords: -
 Abstract: We studied the emission of mutants of the red fluorescent protein DsRed by room temperature single molecule fluorescence spectroscopy. Bulk samples of the DsRed variant E8 show mixed green and red fluorescence of equivalent intensities individually spectrally similar to arrested green and mature red fluorescent forms of DsRed. Investigations at the single molecule level indicate that, like DsRed, E8 is not monomeric at single molecule concentrations. The entities visualized are composed of green and red emitting proteins without a fixed ratio of green to red fluorescing units. We find indications for only weak, if any, fluorescence resonance energy transfer (FRET) between red and green chromophores within one E8 entity. (C) 2002 Elsevier Science B.V. All rights reserved.

Details

show
hide
Language(s): eng - English
 Dates: 2002-08-26
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Chemical Physics Letters
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 362 (5-6) Sequence Number: - Start / End Page: 355 - 361 Identifier: -