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  Comprehensive small animal imaging strategies on a clinical 3 T dedicated head MR-scanner: Adapted methods and sequence protocols in CNS pathologies

Pillai, D. R., Heidemann, R. M., Kumar, P., Shanbhag, N., Lanz, T., Dittmar, M. S., et al. (2011). Comprehensive small animal imaging strategies on a clinical 3 T dedicated head MR-scanner: Adapted methods and sequence protocols in CNS pathologies. PLoS One, 6(2): e16091. doi:10.1371/journal.pone.0016091.

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© 2011 Pillai et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Pillai, Deepu R.1, 2, Author
Heidemann, Robin M.3, 4, Author              
Kumar, Praveen1, 5, Author
Shanbhag, Nagesh1, Author
Lanz, Titus6, Author
Dittmar, Michael S.7, Author
Sander, Beatrice1, Author
Beier, Christoph P.1, 5, Author
Weidner, Norbert1, 8, Author
Greenlee, Mark W.9, Author
Schuierer, Gerhard10, Author
Bogdahn, Ulrich1, Author
Schlachetzki, Felix1, Author
1Regensburg University Medical Centre, Germany, ou_persistent22              
2Julius-Maximilians-University Würzburg, Germany, ou_persistent22              
3Department Neurophysics, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634550              
4Siemens Healthcare Sector, Erlangen, Germany, ou_persistent22              
5University Medical Centre, RWTH Aachen, Germany, ou_persistent22              
6RAPID Biomedical GmbH, Würzburg-Rimpar, Germany, ou_persistent22              
7Department of Anaesthesiology, Regensburg University Medical Centre, Germany, ou_persistent22              
8University of Heidelberg, Germany, ou_persistent22              
9University of Regensburg, Germany, ou_persistent22              
10Regensburger University Medical Centre and Bezirksklinikum Regensburg, Germany, ou_persistent22              


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 Abstract: Background: Small animal models of human diseases are an indispensable aspect of pre-clinical research. Being dynamic, most pathologies demand extensive longitudinal monitoring to understand disease mechanisms, drug efficacy and side effects. These considerations often demand the concomitant development of monitoring systems with sufficient temporal and spatial resolution. Methodology and Results: This study attempts to configure and optimize a clinical 3 Tesla magnetic resonance scanner to facilitate imaging of small animal central nervous system pathologies. The hardware of the scanner was complemented by a custom-built, 4-channel phased array coil system. Extensive modification of standard sequence protocols was carried out based on tissue relaxometric calculations. Proton density differences between the gray and white matter of the rodent spinal cord along with transverse relaxation due to magnetic susceptibility differences at the cortex and striatum of both rats and mice demonstrated statistically significant differences. The employed parallel imaging reconstruction algorithms had distinct properties dependent on the sequence type and in the presence of the contrast agent. The attempt to morphologically phenotype a normal healthy rat brain in multiple planes delineated a number of anatomical regions, and all the clinically relevant sequels following acute cerebral ischemia could be adequately characterized. Changes in blood-brainbarrier permeability following ischemia-reperfusion were also apparent at a later time. Typical characteristics of intracerebral haemorrhage at acute and chronic stages were also visualized up to one month. Two models of rodent spinal cord injury were adequately characterized and closely mimicked the results of histological studies. In the employed rodent animal handling system a mouse model of glioblastoma was also studied with unequivocal results. Conclusions: The implemented customizations including extensive sequence protocol modifications resulted in images of high diagnostic quality. These results prove that lack of dedicated animal scanners shouldn’t discourage conventional small animal imaging studies.


Language(s): eng - English
 Dates: 2010-10-142010-12-092011-02-07
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1371/journal.pone.0016091
 Degree: -



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Title: PLoS One
Source Genre: Journal
Publ. Info: San Francisco, CA : Public Library of Sciene
Pages: - Volume / Issue: 6 (2) Sequence Number: e16091 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850