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  Increased anxiety-related behaviour in Hint1 knockout mice

Varadarajulu, J., Lebar, M., Krishnamoorthy, G., Habelt, S., Lu, J., Weinstein, I. B., et al. (2011). Increased anxiety-related behaviour in Hint1 knockout mice. Behavioural Brain Research, 220(2), 305-311.

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 Creators:
Varadarajulu, J.1, Author
Lebar, M.1, Author
Krishnamoorthy, G.2, Author           
Habelt, S.1, Author
Lu, J.1, Author
Weinstein, I. B.1, Author
Li, H. Y.1, Author
Holsboer, F.1, Author
Turck, C. W.1, Author
Touma, C.1, Author
Affiliations:
1[Varadarajulu, Jeeva; Lebar, Maria; Habelt, Sonja; Lu, Jia; Holsboer, Florian; Turck, Christoph W.; Touma, Chadi] Max Planck Inst Psychiat, Res Grp Prote & Biomarkers, D-80804 Munich, Germany.; [Varadarajulu, Jeeva; Lebar, Maria; Habelt, Sonja; Lu, Jia; Holsboer, Florian; Turck, Christoph W.; Touma, Chadi] Max Planck Inst Psychiat, Res Grp Psychoneuroendocrinol, D-80804 Munich, Germany.; [Weinstein, I. Bernard; Li, Haiyang] Columbia Univ, Med Ctr, Herbert Irving Comprehens Canc Ctr, New York, NY 10027 USA.; [Weinstein, I. Bernard] Columbia Univ, Med Ctr, Dept Med, New York, NY 10027 USA., ou_persistent22              
2Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, ou_1113547              

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Free keywords: Hint1; Elevated plus-maze; Anxiety-related phenotype; Protein kinase C gamma; Biomarker; PKC enzymatic assay
 Abstract: Several reports have implicated a role for the histidine triad nucleotide-binding protein-1 (Hint1) in psychiatric disorders. We have studied the emotional behaviour of male Hint1 knockout (Hint1 KO) mice in a battery of tests and performed biochemical analyses on brain tissue. The behavioural analysis revealed that Hint1 KO mice exhibit an increased emotionality phenotype compared to wildtype (WT) mice, while no significant differences in locomotion or general exploratory activity were noted. In the elevated plus-maze (EPM) test, the Hint1 KO animals entered the open arms of the apparatus less often than WT littermates. Similarly, in the dark-light box test, Hint1 KO mice spent less time in the lit compartment and the number of entries were reduced, which further confirmed an increased anxiety-related behaviour. Moreover, the Hint1 KO animals showed significantly more struggling and less floating behaviour in the forced swim test (FST), indicating an increased emotional arousal in aversive situations. Hint1 is known as a protein kinase C (PKC) interacting protein. Western blot analysis showed that PKC gamma expression was elevated in Hint1 KO compared to WT mice. Interestingly, PKC-gamma mRNA levels of the two groups did not show a significant difference, implying a post-transcriptional PKC-gamma regulation. In addition, PKC enzymatic activity was increased in Hint1 KO compared to WT mice. In summary, our results indicate a role for Hint1 and PKC gamma in modulating anxiety-related and stress-coping behaviour in mice. (C) 2011 Elsevier B.V. All rights reserved.

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Language(s): eng - English
 Dates: 2011-07-07
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 563259
ISI: 000289958300006
 Degree: -

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Title: Behavioural Brain Research
  Alternative Title : Behav. Brain Res.
Source Genre: Journal
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Publ. Info: AMSTERDAM : ELSEVIER SCIENCE BV
Pages: - Volume / Issue: 220 (2) Sequence Number: - Start / End Page: 305 - 311 Identifier: ISSN: 0166-4328