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  Monitoring calcium levels with genetically encoded indicators

Garaschuk, O., & Griesbeck, O. (2009). Monitoring calcium levels with genetically encoded indicators. In A. Verkhratsky, & O. Petersen (Eds.), Calcium Measurement Methods (pp. 101-107). New York, Dordrecht, Heidelberg, London: Humana Press, Springer.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0012-201D-B Version Permalink: https://hdl.handle.net/21.11116/0000-000D-C158-6
Genre: Book Chapter

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 Creators:
Garaschuk, O., Author
Griesbeck, O.1, Author           
Affiliations:
1Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society, ou_1113560              

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 Abstract: Calcium indicators are widely used to monitor activity in living neuronal tissue because of the tight relation between action potential firing and increases in the intracellular calcium concentration. Here, we describe the use of genetically encoded calcium indicators (GECIs) of the latest generation for monitoring calcium levels in the mammalian brain. We discuss how to choose the sensor for a given experiment, how to introduce the sensor into the cells of interest and how to estimate the sensitivity of the sensor in situ and in vivo. Finally, we illustrate the application of these sensors for high resolution in vivo imaging of sensory-driven neuronal activity.

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Language(s): eng - English
 Dates: 2009-12-02
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 444308
DOI: 10.1007/978-1-60761-476-0_5
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Title: Calcium Measurement Methods
Source Genre: Book
 Creator(s):
Verkhratsky, A., Editor
Petersen, O.H., Editor
Affiliations:
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Publ. Info: New York, Dordrecht, Heidelberg, London : Humana Press, Springer
Pages: - Volume / Issue: 43 Sequence Number: - Start / End Page: 101 - 107 Identifier: ISBN: 978-1-60761-476-0
ISBN: 978-1-60761-475-3

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Title: Neuromethods
Source Genre: Series
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Pages: - Volume / Issue: 43 Sequence Number: - Start / End Page: - Identifier: -