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  The Presence of Lytic HSV-1 Transcripts and Clonally Expanded T Cells with a Memory Effector Phenotype in Human Sensory Ganglia

Derfuss, T., Arbusow, V., Strupp, M., Brandt, T., & Theil, D. (2009). The Presence of Lytic HSV-1 Transcripts and Clonally Expanded T Cells with a Memory Effector Phenotype in Human Sensory Ganglia. In Strupp, Michael, & al. (Eds.), BASIC AND CLINICAL ASPECTS OF VERTIGO AND DIZZINESS (pp. 300-304). OXFORD: BLACKWELL PUBLISHING.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-20CE-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-20CF-E
Genre: Book Chapter

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 Creators:
Derfuss, T.1, Author              
Arbusow, V.2, Author
Strupp, M.2, Author
Brandt, T.2, Author
Theil, D.2, Author
Affiliations:
1Department: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, ou_1113547              
2[Arbusow, Viktor; Strupp, Michael; Brandt, Thomas; Theil, Diethilde] Univ Munich, Klinikum Grosshadern, Dept Neurol, D-81377 Munich, Germany.; [Derfuss, Tobias] Univ Munich, Inst Clin Neuroimmunol, D-81377 Munich, Germany., ou_persistent22              

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Free keywords: sensory ganglia; HSV-1; T cells; lytic transcripts; clonal expansion; cranial nerve disorders
 Abstract: Herpes simplex virus type 1 (HSV-1) latent persistence in human trigeminal ganglia (TG) is accompanied by a chronic CD8 T-cell infiltration. Thus far, during HSV-1 latency only a single transcript, namely the latency-associated transcript (LAT), has been identified to be synthesized but not translated into a protein. In contrast, the chronic CD8 T-cell infiltration suggests that an antigen trigger must be present. The focus of the current work was to look for HSV-1 transcription activity as a potential trigger of the immune response and to demonstrate whether the immune cells are clonally expanded and have a phenotype that suggests that they have been triggered by viral antigen. By combining in situ hybridization, laser cutting microscopy, and single-cell real time RT-PCR, we demonstrated expression of the HSV-1 immediate early (IE) genes ICP0 and ICP4 in human trigeminal neurons. Using CDR3 spectratyping, we showed that the infiltrating T cells are clonally expanded, indicating an antigen-driven immune response. Moreover, the persisting CD8(+) T cells had prominent features of the memory effector phenotype. Chemokines CCL5 and CXCL10 were expressed by a subpopulation of infiltrating cells and the corresponding chemokine receptors CCR5 and CXCR3 were co-expressed on virtually all T cells bearing the CD8 phenotype. Thus, HSV-1 IE genes are expressed in human TG, and the infiltrating T cells bear several characteristics that suggest viral antigenic stimulation.

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Language(s): eng - English
 Dates: 2009
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: eDoc: 432416
ISI: 000267019700042
 Degree: -

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Title: BASIC AND CLINICAL ASPECTS OF VERTIGO AND DIZZINESS
Source Genre: Book
 Creator(s):
Strupp, Editor
Michael, Editor
al., Editor
Affiliations:
-
Publ. Info: OXFORD : BLACKWELL PUBLISHING
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 300 - 304 Identifier: -

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Title: Annals of the New York Academy of Sciences
Source Genre: Series
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Pages: - Volume / Issue: 1164 Sequence Number: - Start / End Page: - Identifier: ISSN: 0077-8923