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  Modification of choline acetyltransferase by integration of green fluorescent protein does not affect enzyme activity and subcellular distribution

Rathenberg, J., Gärtner, A., Koenen, M., & Witzemann, V. (2002). Modification of choline acetyltransferase by integration of green fluorescent protein does not affect enzyme activity and subcellular distribution. Cell and Tissue Research, 308(1), 1-6.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0012-237C-0 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0012-237C-0
Genre: Journal Article

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 Creators:
Rathenberg, J.1, Author
Gärtner, A.2, Author           
Koenen, M.1, Author
Witzemann, V.1, Author
Affiliations:
1Max Planck Inst Med Res, Zellphysiol Abt, Jahnstr 29, D-69120; Heidelberg, Germany; Max Planck Inst Med Res, Zellphysiol Abt, D-69120 Heidelberg, Germany;, ou_persistent22              
2Emeritus Group: Neurochemistry / Thoenen, MPI of Neurobiology, Max Planck Society, ou_1113550              

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Free keywords: ChAT; GFP; cholinergic marker; in vivo visualization; mouse (C57/B16); cell culture; rat (Wistar)
 Abstract: Choline acetyltransferase (ChAT) is widely used as a marker enzyme to identify cholinergic neurons in the central and peripheral nerve us system and to study developmental changes. In order to visualize expression of ChAT directly we have generated a ChAT-green fluorescent protein (GFP) fusion construct. Upon transfection of COS-1 cells and cultured rat hippocampal neurons, transgenic enzymatically active ChAT-GFP is expressed and shows intrinsic fluorescence. In COS-1 cells the ChAT-GFP construct revealed a subcellular distribution indistinguishable from wild-type ChAT. In primary neurons the fluorescence was present in the soma and neuritic processes. Hence, this construct will be useful for analyzing the expression and subcellular distribution of ChAT-GFP in cell and tissue culture.

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Language(s): eng - English
 Dates: 2002-04
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 27061
ISI: 000175869000001
 Degree: -

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Title: Cell and Tissue Research
  Alternative Title : Cell Tissue Res.
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 308 (1) Sequence Number: - Start / End Page: 1 - 6 Identifier: ISSN: 0302-766X