English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Integration of a barley (Hordeum vulgare) molecular linkage, map with the position of genetic loci hosting 29 developmental mutants

Pozzi, C., di Pietro, D., Halas, G., Roig, C., & Salamini, F. (2003). Integration of a barley (Hordeum vulgare) molecular linkage, map with the position of genetic loci hosting 29 developmental mutants. Heredity, 90(5), 390-396.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3CD5-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3CD7-E
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Pozzi, C.1, Author              
di Pietro, D., Author
Halas, G., Author
Roig, C., Author
Salamini, F.1, Author              
Affiliations:
1Dept. of Plant Breeding and Yield Physiology (Francesco Salamini), MPI for Plant Breeding Research, Max Planck Society, ou_1113570              

Content

show
hide
Free keywords: linkage map; mutant mapping; AFLP
 Abstract: The first step in positional gene cloning is the integration into available molecular maps of genetic loci for which mutant alleles exist. We report the placement of 29 barley developmental mutants on a restriction fragment length polymorphism-amplified fragment length polymorphism (RFLP-AFLP) map. The mapping procedure used homozygous mutant F-2 plants in an iterative process: once a mutant linked AFLP was found, primer combinations were successively selected to generate AFLP fragments more tightly linked to the mutant locus. The mutants considered were ado, als, aur-a1, aur-a2, br1, br2, bra-d7, cul3, cul5, cul15, cul16, den6, den8, dub1, hex-v3, hex-v4, int-c5, K, li, lig-a2, lk2, lk5, sld1, sld4, tr, trd, unc, uc2 and uz. The 29 mutant loci were linked to the closest molecular markers by distances ranging from 0 to 23 cM, with an average value of 3.8 cM away. Since the efficiency of the mapping procedure is a function of the density of molecular markers, the RFLP-AFLP map of Castiglioni et al was further integrated with new AFLPs using 87 doubled haploid lines derived from the barley cross Igri x Danilo. A total of 819 mapped AFLP marker loci are now available in the combined map.

Details

show
hide
Language(s): eng - English
 Dates: 2003-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28741
ISI: 000182910800008
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Heredity
  Alternative Title : Heredity
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 90 (5) Sequence Number: - Start / End Page: 390 - 396 Identifier: ISSN: 0018-067X