User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse


  Barley disease resistance gene analogs of the NBS-LRR class: identification and mapping

Madsen, L. H., Collins, N. C., Rakwalska, M., Backes, G., Sandal, N., Krusell, L., et al. (2003). Barley disease resistance gene analogs of the NBS-LRR class: identification and mapping. Molecular Genetics and Genomics, 269(1), 150-161.

Item is


show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3CE3-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3CE4-0
Genre: Journal Article


show Files




Madsen, L. H., Author
Collins, N. C., Author
Rakwalska, M., Author
Backes, G., Author
Sandal, N., Author
Krusell, L., Author
Jensen, J., Author
Waterman, E. H., Author
Jahoor, A., Author
Ayliffe, M., Author
Pryor, A. J., Author
Langridge, P., Author
Schulze-Lefert, P.1, Author              
Stougaard, J., Author
1Dept. of Plant Microbe Interactions (Paul Schulze-Lefert), MPI for Plant Breeding Research, Max Planck Society, ou_1113572              


Free keywords: barley; disease resistance gene analogs (RGAs); NBS-LRR proteins; mapping
 Abstract: The majority of verified plant disease resistance genes isolated to date are of the NBS-LRR class, encoding proteins with a predicted nucleotide binding site (NBS) and a leucine- rich repeat (LRR) region. We took advantage of the sequence conservation in the NBS motif to clone, by PCR, gene fragments from barley representing putative disease resistance genes of this class. Over 30 different resistance gene analogs (RGAs) were isolated from the barley cultivar Regatta. These were grouped into 13 classes based on DNA sequence similarity. Actively transcribed genes were identified from all classes but one, and cDNA clones were isolated to derive the complete NBS- LRR protein sequences. Some of the NBS-LRR genes exhibited variation with respect to whether and where particular introns were spliced, as well as frequent premature polyadenylation. DNA sequences related to the majority of the barley RGAs were identified in the recently expanded public rice genomic sequence database, indicating that. the rice sequence can be used to extract a large proportion of the RGAs from barley and other cereals. Using a combination of RFLP and PCR marker techniques, representatives of all barley RGA gene classes were mapped in the barley genome, to all chromosomes except 4H. A number of the RGA loci map in the vicinity of known disease resistance loci, and the association between RGA S-120 and the nematode resistance locus Ha2on chromosome 2H was further tested by co-segregation analysis. Most of the RGA sequences reported here have not been described previously, and represent a useful resource as candidates or molecular markers for disease resistance genes in barley and other cereals. Electronic Supplementary Material Supplementary material is available for this article if you access the article at http://dx.doi.org/10.1007/s00438-003-0823-5. A link in the frame on the left on that,page takes you directly to the supplementary material.


Language(s): eng - English
 Dates: 2003-04
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28724
ISI: 000183032400016
 Degree: -



Legal Case


Project information


Source 1

Title: Molecular Genetics and Genomics
  Alternative Title : Mol. Genet. Genomics
Source Genre: Journal
Publ. Info: -
Pages: - Volume / Issue: 269 (1) Sequence Number: - Start / End Page: 150 - 161 Identifier: ISSN: 1617-4615