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  Multi-functional T-DNA/Ds tomato lines designed for gene cloning and molecular and physical dissection of the tomato genome

Gidoni, D., Fuss, E., Burbidge, A., Speckmann, G. J., James, S., Nijkamp, D., et al. (2003). Multi-functional T-DNA/Ds tomato lines designed for gene cloning and molecular and physical dissection of the tomato genome. Plant Molecular Biology, 51(1), 83-98.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3D15-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3D16-7
Genre: Journal Article

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 Creators:
Gidoni, D., Author
Fuss, E., Author
Burbidge, A., Author
Speckmann, G. J., Author
James, S., Author
Nijkamp, D., Author
Mett, A., Author
Feiler, J., Author
Smoker, M., Author
de Vroomen, M. J., Author
Leader, D., Author
Liharska, T., Author
Groenendijk, J., Author
Coppoolse, E., Author
Smit, J. J. M., Author
Levin, I., Author
de Both, M., Author
Schuch, W., Author
Jones, J. D. G., Author
Taylor, I. B., Author
Theres, K.1, Author              van Haaren, M. J. J., Author more..
Affiliations:
1Dept. of Plant Breeding and Genetics (Maarten Koornneef), MPI for Plant Breeding Research, Max Planck Society, ou_1113569              

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Free keywords: chromosomal rearrangements; site-specific recombination; T-DNA; tomato; transposon
 Abstract: In order to make the tomato genome more accessible for molecular analysis and gene cloning, we have produced 405 individual tomato (Lycopersicon esculentum) lines containing a characterized copy of pJasm13, a multifunctional T-DNA/modified Ds transposon element construct. Both the T-DNA and the Ds element in pJasm13 harbor a set of selectable marker genes to monitor excision and reintegration of Ds and additionally, target sequences for rare cutting restriction enzymes (I-PpoI, SfiI, NotI) and for site-specific recombinases (Cre, FLP, R). Blast analysis of flanking genomic sequences of 174 T-DNA inserts revealed homology to transcribed genes in 69 (40%), of which about half are known or putatively identified as genes and ESTs. The map position of 140 individual inserts was determined on the molecular genetic map of tomato. These inserts are distributed over the 12 chromosomes of tomato, allowing targeted and non-targeted transposon tagging, marking of closely linked genes of interest and induction of chromosomal rearrangements including translocations or creation of saturation-deletions or inversions within defined regions linked to the T-DNA insertion site. The different features of pJasm13 were successfully tested in tomato and Arabidopsis thaliana, thus providing a new tool for molecular/genetic dissection studies, including molecular and physical mapping, mutation analysis and cloning strategies in tomato and potentially, in other plants as well.

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Language(s): eng - English
 Dates: 2003-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 127745
ISI: 000178667200008
 Degree: -

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Title: Plant Molecular Biology
  Alternative Title : Plant Mol.Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 51 (1) Sequence Number: - Start / End Page: 83 - 98 Identifier: ISSN: 0167-4412