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  Knock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency accompanied by cell differentiation defects

Henriques, R., Jasik, J., Klein, M., Martinoia, E., Feller, U., Schell, J., et al. (2002). Knock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency accompanied by cell differentiation defects. Plant Molecular Biology, 50(4), 587-597.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3D54-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3D55-8
Genre: Journal Article

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 Creators:
Henriques, R., Author
Jasik, J.1, 2, Author              
Klein, M., Author
Martinoia, E., Author
Feller, U., Author
Schell, J.2, Author              
Pais, M. S., Author
Koncz, C.1, Author              
Affiliations:
1Dept. of Plant Developmental Biology (George Coupland), MPI for Plant Breeding Research, Max Planck Society, ou_1113571              
2Dept. of Genetic Principles of Plant Breeding (Jozef Schell), MPI for Plant Breeding Research, Max Planck Society, ou_1113567              

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Free keywords: Arabidopsis; insertion mutagenesis; IRT; metal-transporter; ZIP
 Abstract: IRT1 and IRT2 are members of the Arabidopsis ZIP metal transporter family that are specifically induced by iron deprivation in roots and act as heterologous suppressors of yeast mutations inhibiting iron and zinc uptake. Although IRT1 and IRT2 are thought to perform redundant functions as root- specific metal transporters, insertional inactivation of the IRT1 gene alone results in typical symptoms of iron deficiency causing severe leaf chlorosis and lethality in soil. The irt1 mutation is characterized by specific developmental defects, including a drastic reduction of chloroplast thylakoid stacking into grana and lack of palisade parenchyma differentiation in leaves, reduced number of vascular bundles in stems, and irregular patterns of enlarged endodermal and cortex cells in roots. Pulse labeling with (5)9Fe through the root system shows that the irt1 mutation reduces iron accumulation in the shoots. Short-term labeling with (6)5Zn reveals no alteration in spatial distribution of zinc, but indicates a lower level of zinc accumulation. In comparison to wild-type, the irt1 mutant responds to iron and zinc deprivation by altered expression of certain zinc and iron transporter genes, which results in the activation of ZIP1 in shoots, reduction of ZIP2 transcript levels in roots, and enhanced expression of IRT2 in roots. These data support the conclusion that IRT1 is an essential metal transporter required for proper development and regulation of iron and zinc homeostasis in Arabidopsis.

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Language(s): eng - English
 Dates: 2002-11
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28790
ISI: 000177679200001
 Degree: -

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Title: Plant Molecular Biology
  Alternative Title : Plant Mol.Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 50 (4) Sequence Number: - Start / End Page: 587 - 597 Identifier: ISSN: 0167-4412