English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Primary structure and tissue-specific expression of the pathogenesis-related protein PR-1b in potato

Hoegen, E., Stromberg, A., Pihlgren, U., & Kombrink, E. (2002). Primary structure and tissue-specific expression of the pathogenesis-related protein PR-1b in potato. Molecular Plant Pathology, 3(5), 329-345.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3D84-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3D85-B
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hoegen, E., Author
Stromberg, A., Author
Pihlgren, U., Author
Kombrink, E.1, Author              
Affiliations:
1Dept. of Plant Microbe Interactions (Paul Schulze-Lefert), MPI for Plant Breeding Research, Max Planck Society, ou_1113572              

Content

show
hide
Free keywords: -
 Abstract: The infection of potato (Solanum tuberosum) leaves with the late blight pathogen Phytophthora infestans, or treatment with fungal elicitor, leads to the massive accumulation of pathogenesis-related (PR) proteins in the extracellular leaf space. The most abundant of these proteins was purified to apparent homogeneity and identified as a new, basic member of the PR-1 family of defence proteins, designated PR-1b. Antibodies raised against the protein and a cDNA isolated by differential screening were used to study the temporal and spatial patterns of PR-1b protein and mRNA distribution in healthy and infected potato tissues. PR-1b was present in old leaves and at low levels also in the carpels of flowers, In leaves, strong accumulation of PR-1b mRNA and protein occurred in response to infection by the oomycete pathogen Phytophthora intestans or the bacterial pathogen Pseudomonas syringae pv. maculicola, PR-1b mRNA and protein accumulation was clearly initiated at the infection site, but a delayed and sustained accumulation was also observed in neighbouring, uninfected leaves of potato plants. Tissue- and cell type-specific expression of PR-1b was analysed by immunohistochemical and in situ RNA hybridization techniques. Appreciable amounts of PR-1b protein and mRNA were localized in epidermal cells, guard cells of the stomata, glandular trichomes, crystal idioblasts, and cells of the vascular system of infected leaves. However, no significant differences in the amounts and distribution patterns of PR-1b could be observed between compatible and incompatible interactions of potato and Phytophthora infestans, indicating that PR-1b expression is not involved in determining cultivar/ race-specific resistance in potato.

Details

show
hide
Language(s): eng - English
 Dates: 2002-09
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28781
ISI: 000178157500005
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Plant Pathology
  Alternative Title : Mol. Plant Pathol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 3 (5) Sequence Number: - Start / End Page: 329 - 345 Identifier: ISSN: 1464-6722