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  Antagonistic regulation of flowering-time gene SOC1 by CONSTANS and FLC via separate promoter motifs

Hepworth, S. R., Valverde, F., Ravenscroft, D., Mouradov, A., & Coupland, G. (2002). Antagonistic regulation of flowering-time gene SOC1 by CONSTANS and FLC via separate promoter motifs. EMBO Journal, 21(16), 4327-4337.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3D9E-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3D9F-4
Genre: Journal Article

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 Creators:
Hepworth, S. R., Author
Valverde, F.1, Author              
Ravenscroft, D.1, Author              
Mouradov, A.1, Author              
Coupland, G.1, Author              
Affiliations:
1Dept. of Plant Developmental Biology (George Coupland), MPI for Plant Breeding Research, Max Planck Society, ou_1113571              

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Free keywords: Arabidopis; CONSTANS; FLC; flowering; SOC1
 Abstract: Flowering in Arabidopsis is controlled by endogenous and environmental signals relayed by distinct genetic pathways. The MADS-box flowering-time gene SOC1 is regulated by several pathways and is proposed to co-ordinate responses to environmental signals. SOC1 is directly activated by CONSTANS (CO) in long photoperiods and is repressed by FLC, a component of the vernalization (low-temperature) pathway. We show that in transgenic plants overexpressing CO and FLC, these proteins regulate flowering time antagonistically and FLC blocks transcriptional activation of SOC1 by CO. A series of SOC1::GUS reporter genes identified a 351 bp promoter sequence that mediates activation by CO and repression by FLC. A CArG box (MADS-domain protein binding element) within this sequence was recognized specifically by FLC in vitro and mediated repression by FLC in vivo, suggesting that FLC binds directly to the SOC1 promoter. We propose that CO is recruited to a separate promoter element by a DNA-binding factor and that activation by CO is impaired when FLC is bound to an adjacent CArG motif.

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Language(s): eng - English
 Dates: 2002-08-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28786
ISI: 000177355300013
 Degree: -

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Title: EMBO Journal
  Alternative Title : Embo J.
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 21 (16) Sequence Number: - Start / End Page: 4327 - 4337 Identifier: ISSN: 0261-4189