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  A test of fusion protein stability in the plant Arabidopsis thaliana reveals degradation signals from ACC synthase and from the plant N-end rule pathway

Schlogelhofer, P., & Bachmair, A. (2002). A test of fusion protein stability in the plant Arabidopsis thaliana reveals degradation signals from ACC synthase and from the plant N-end rule pathway. Plant Cell Reports, 21(2), 174-179.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DA0-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DA1-D
Genre: Journal Article

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 Creators:
Schlogelhofer, P.1, Author              
Bachmair, A.1, Author              
Affiliations:
1Dept. of Plant Developmental Biology (George Coupland), MPI for Plant Breeding Research, Max Planck Society, ou_1113571              

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Free keywords: ACC synthase; deg1; clasto-lactacystin-beta-lactone; N-end rule; protein turnover
 Abstract: We tested three different protein domains for their ability to confer a short half life onto the otherwise stable test protein dihydrofolate reductase (DHFR) in the plant Arabidopsis thaliana. E-DHFR, a protein with Glu as the first residue, is metabolically unstable in Arabidopsis, indicating that Glu is a destabilizing residue of the plant N-end rule pathway. In contrast, the degradation signal deg1 from Saccharomyces cerevisiae transcription factor Matalpha2p is not recognized when present at the amino-terminus of DHFR. Finally, we show that the amino-terminal 93 amino acids of the plant protein 1- aminocyclopropane-1-carboxylic acid synthase contain a degradation signal that is also active in yeast.

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Language(s): eng - English
 Dates: 2002-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28784
ISI: 000178070900012
 Degree: -

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Title: Plant Cell Reports
  Alternative Title : Plant Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 21 (2) Sequence Number: - Start / End Page: 174 - 179 Identifier: ISSN: 0721-7714