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  Simple and versatile selection of Arabidopsis transformants

Hadi, M. Z., Kemper, E., Wendeler, E., & Reiss, B. (2002). Simple and versatile selection of Arabidopsis transformants. Plant Cell Reports, 21(2), 130-135.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DA2-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DA3-9
Genre: Journal Article

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 Creators:
Hadi, M. Z., Author
Kemper, E., Author
Wendeler, E.1, Author              
Reiss, B.1, Author              
Affiliations:
1Dept. of Genetic Principles of Plant Breeding (Jozef Schell), MPI for Plant Breeding Research, Max Planck Society, ou_1113567              

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Free keywords: transformation; selectable markers; universal selection system; greenhouse
 Abstract: The selection of large amounts of seeds of Arabidopsis thaliana obtained after in planta transformation under sterile conditions and in tissue culture is relatively labour- intensive, time-consuming and expensive. We describe here an alternative selection procedure that uses regular greenhouse conditions and functions with a wide variety of selective agents. The selection system is based on the inert growth substrate Grodan and was intensively tested for the selection of transgenic Arabidopsis seeds with kanamycin, hygromycin, BASTA, sulfadiazine and methotrexate. However, the system has the capacity to be applicable for more selective agents and to a wider range of plant species. For Arabidopsis, the effectiveness of selective agents is comparable to or better than that obtained with tissue culture procedures.

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Language(s): eng - English
 Dates: 2002-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28785
ISI: 000178070900005
 Degree: -

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Title: Plant Cell Reports
  Alternative Title : Plant Cell Reports
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 21 (2) Sequence Number: - Start / End Page: 130 - 135 Identifier: ISSN: 0721-7714