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  Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley

Cormack, R. S., Eulgem, T., Rushton, P. J., Koechner, P., Hahlbrock, K., & Somssich, I. E. (2002). Leucine zipper-containing WRKY proteins widen the spectrum of immediate early elicitor-induced WRKY transcription factors in parsley. Biochimica et Biophysica Acta-Gene Structure and Expression, 1576(1-2), 92-100.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DC7-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DC8-8
Genre: Journal Article

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 Creators:
Cormack, R. S.1, Author              
Eulgem, T.1, Author              
Rushton, P. J.2, Author              
Koechner, P.2, Author              
Hahlbrock, K.1, Author              
Somssich, I. E.2, Author              
Affiliations:
1Dept. of Biochemistry (Klaus Hahlbrock), MPI for Plant Breeding Research, Max Planck Society, ou_1113566              
2Dept. of Plant Microbe Interactions (Paul Schulze-Lefert), MPI for Plant Breeding Research, Max Planck Society, ou_1113572              

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Free keywords: protein synthesis inhibitor study; transient transfection assay; yeast one-hybrid screen; WRKY transcription factor
 Abstract: Two new WRKY transcription factors from parsley (Petroselinum crispum), WRKY4 and WRKY5, were isolated using the yeast one- hybrid system. In yeast, both proteins interacted sequence- specifically with W boxes (TTGACC) and activated transcription. They appear to contain functional leucine zippers, which increase their affinities for W boxes. Co-transfection experiments in parsley protoplasts confirmed their in vivo- binding specificity for W boxes. Elicitor-mediated expression of the WRKY5 gene, the first parsley member of the group III family of WRKY proteins, is extremely transient, with high mRNA levels occurring within a time window of less than I h. WRKY4 and -5, as well as the previously identified parsley transcription factors WRKY 1 and -3, are encoded by immediate early elicitor-activated genes that differ in their sensitivity to cycloheximide (CHX) and their activation kinetics. We propose that a number of the pathways activated during the plant defense response require the induction of several distinct WRKY transcription factors with different DNA binding- site preferences to fine-tune the activation of a wide spectrum of target genes. (C) 2002 Elsevier Science B.V. All rights reserved.

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Language(s): eng - English
 Dates: 2002-06-07
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 127496
ISI: 000176060700011
 Degree: -

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Title: Biochimica et Biophysica Acta-Gene Structure and Expression
  Alternative Title : Biochim. Biophys. Acta-Gene Struct. Expression
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1576 (1-2) Sequence Number: - Start / End Page: 92 - 100 Identifier: ISSN: 0167-4781