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  Single and double knockouts of the genes for photosystem I subunits G, K, and H of Arabidopsis. Effects on photosystem I composition, photosynthetic electron flow, and state transitions

Varotto, C., Pesaresi, P., Jahns, P., Lessnick, A., Tizzano, M., Schiavon, F., et al. (2002). Single and double knockouts of the genes for photosystem I subunits G, K, and H of Arabidopsis. Effects on photosystem I composition, photosynthetic electron flow, and state transitions. Plant Physiology, 129(2), 616-624.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DCC-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DCE-B
Genre: Journal Article

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 Creators:
Varotto, C.1, 2, Author              
Pesaresi, P.1, Author              
Jahns, P., Author
Lessnick, A., Author
Tizzano, M., Author
Schiavon, F., Author
Salamini, F.1, Author              
Leister, D.1, Author              
Affiliations:
1Dept. of Plant Breeding and Yield Physiology (Francesco Salamini), MPI for Plant Breeding Research, Max Planck Society, ou_1113570              
2Dept. of Molecular Plant Genetics (Heinz Saedler), MPI for Plant Breeding Research, Max Planck Society, ou_1113568              

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 Abstract: Photosystem I (PSI) of higher plants contains 18 subunits. Using Arabidopsis En insertion lines, we have isolated knockout alleles of the genes psaG, psaH2, and psaK, which code for PSI- G, -H, and -K. In the mutants psak-1 and psag-1.4, complete loss of PSI-K and -G, respectively, was confirmed, whereas the residual H level in psah2-1.4 is due to a second gene encoding PSI-H, psaH1. Double mutants, lacking PSI-G, and also -K, or a fraction of -H, together with the three single mutants were characterized for their growth phenotypes and PSI polypeptide composition. In general, the loss of each subunit has secondary, in some cases additive, effects on the abundance of other PSI polypeptides, such as D, E, H, L, N, and the light- harvesting complex I proteins Lhca2 and 3. In the G-less mutant psag-1.4, the variation in PSI composition suggests that PSI-G stabilizes the PSI-core. Levels of light-harvesting complex I proteins in plants, which lack simultaneously PSI-G and -K, indicate that PSI subunits other than G and K can also bind Lhca2 and 3. In the same single and double mutants, psag-1.4, psak-1, psah2-1.4, psag-1.4/psah2-1.4, and psag-1.4/psak-1 photosynthetic electron flow and excitation energy quenching were analyzed to address the roles of the various subunits in P700 reduction (mediated by PSI-F and -N) and oxidation (PSI- E), and state transitions (PSI-H). Based on the results, we also suggest for PSI-K a role in state transitions.

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Language(s): eng - English
 Dates: 2002-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28800
ISI: 000176363800021
 Degree: -

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Title: Plant Physiology
  Alternative Title : Plant Physiol.
Source Genre: Journal
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Pages: - Volume / Issue: 129 (2) Sequence Number: - Start / End Page: 616 - 624 Identifier: ISSN: 0032-0889