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  Gene-sequence-tag expression analyses of 1,800 genes related to chloroplast functions

Kurth, J., Varotto, C., Pesaresi, P., Biehl, A., Richly, E., Salamini, F., et al. (2002). Gene-sequence-tag expression analyses of 1,800 genes related to chloroplast functions. Planta, 215(1), 101-109.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DDC-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DDE-7
Genre: Journal Article

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 Creators:
Kurth, J., Author
Varotto, C.1, 2, Author              
Pesaresi, P.1, Author              
Biehl, A.3, Author              
Richly, E.1, Author              
Salamini, F.1, Author              
Leister, D.1, Author              
Affiliations:
1Dept. of Plant Breeding and Yield Physiology (Francesco Salamini), MPI for Plant Breeding Research, Max Planck Society, ou_1113570              
2Dept. of Molecular Plant Genetics (Heinz Saedler), MPI for Plant Breeding Research, Max Planck Society, ou_1113568              
3Dept. of Plant Breeding and Genetics (Maarten Koornneef), MPI for Plant Breeding Research, Max Planck Society, ou_1113569              

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Free keywords: Arabidopsis; chloroplast; DNA array; gene sequence tag; photosynthesis
 Abstract: Quantification of the expression levels of nuclear genes encoding plastid proteins under different genetic or environmental conditions can contribute to the genetic dissection of plastid functions. To facilitate such measurements, a set of 1,827 Arabidopsis thaliana genes coding for plastid proteins was PCR-amplified from genomic DNA and spotted on nylon membranes to generate an array of chloroplast- specific gene-sequence-tags (GSTs). The sensitivity and reliability of the experimental system was evaluated and a procedure was developed for detecting differential gene expression. The GST array was found to serve as a reliable monitor of changes in gene expression induced by environmental and genetic alteration of chloroplast functions. Based on comparisons of dark-versus light-grown seedlings, and wild-type versus prpl11-1 plants, lists of differentially expressed genes are provided which include 193/7 and 25/42 up/down-regulated genes, respectively. The cut-off values for differential expression were 2.5-times (up) and 0.40 (down). Additional up- regulated genes with relatively low expression ratios (from 1.5- to 2.5-times) or down-regulated with relatively high ratios (0.4-0.67) can be accessed at the website: http://www- mpiz.mpg.de/similar torichly/GST-array.html. A sample of genes analysed by quantitative reverse transcription PCR confirmed the expression profiles monitored by the GST array. Differential hybridisation experiments with the prpl11-1 mutant revealed the existence of regulatory networks sensing the protein state of the chloroplast and transmitting the signal to the nucleus.

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Language(s): eng - English
 Dates: 2002-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28798
ISI: 000176087900011
 Degree: -

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Title: Planta
  Alternative Title : Planta
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 215 (1) Sequence Number: - Start / End Page: 101 - 109 Identifier: ISSN: 0032-0935