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  Evolution of class B floral homeotic proteins: Obligate heterodimerization originated from homodimerization

Winter, K. U., Weiser, C., Kaufmann, K., Bohne, A., Kirchner, C., Kanno, A., et al. (2002). Evolution of class B floral homeotic proteins: Obligate heterodimerization originated from homodimerization. Molecular Biology and Evolution, 19(5), 587-596.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DEB-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DEC-7
Genre: Journal Article

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 Creators:
Winter, K. U.1, Author              
Weiser, C., Author
Kaufmann, K., Author
Bohne, A., Author
Kirchner, C., Author
Kanno, A., Author
Saedler, H.1, Author              
Theissen, G.1, Author              
Affiliations:
1Dept. of Molecular Plant Genetics (Heinz Saedler), MPI for Plant Breeding Research, Max Planck Society, ou_1113568              

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Free keywords: MADS-box gene; homeotic gene; gymnosperm; angiosperm; evolution; dimerization
 Abstract: The class B floral homeotic genes from the higher eudicot model systems Arabidopsis and Antirrhinum are involved in specifying the identity of petals and stamens during flower development. These genes exist in two different types termed DEF- and GLO- like genes. The proteins encoded by the class B genes are stable and functional in the cell only as heterodimeric complexes of a DEF- and a GLO-like protein. In line with this. heterodimerization is obligate for DNA binding in vitro. The genes whose products have to heterodimerize to be stable and functional are each other's closest relatives within their genomes. This suggests that the respective genes originated by gene duplication. and that heterodimerization is of relative recent origin and evolved from homodimerization. To test this hypothesis we hay e investigated the dimerization behavior of putative B proteins from phylogenetic informative taxa, employing electrophoretic mobility shift assays and the yeast two-hybrid system. We find that an ancestral B protein from the gymnosperm Gnetum gnemon birds DNA in a sequence-specific manner as a homodimer. Of the two types of B proteins from the monocot Lilium regale. the GLO-like protein is still able to homodimerize, whereas the DEF-like protein binds to DNA only as a heterodimeric complex with the GLO-like protein. These data suggest that heterodimerization evolved in two steps after a gene duplication that gave rise to DEF- and GLO-like genes. Heterodimerization may have originated after the gymnosperm- angiosperm split about 300 MYA but before the monocot-eudicot split 140-200 MYA. Heterodimerization may have become obligate for both types of flowering plant B proteins in the eudicot lineage after the monocot-eudicot split.

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Language(s): eng - English
 Dates: 2002-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: eDoc: 28812
ISI: 000175605900001
 Degree: -

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Title: Molecular Biology and Evolution
  Alternative Title : Mol. Biol. Evol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 19 (5) Sequence Number: - Start / End Page: 587 - 596 Identifier: ISSN: 0737-4038