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  Expression of nitrate transporter genes in tomato colonized by an arbuscular mycorrhizal fungus

Hildebrandt, U., Schmelzer, E., & Bothe, H. (2002). Expression of nitrate transporter genes in tomato colonized by an arbuscular mycorrhizal fungus. Physiologia Plantarum, 115(1), 125-136.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DED-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0012-3DEE-3
Genre: Journal Article

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Hildebrandt, U.1, Author
Schmelzer, E.2, 3, Author              
Bothe, H., Author
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2Central Microscopy, MPI for Plant Breeding Research, Max Planck Society, ou_1113565              
3Dept. of Biochemistry (Klaus Hahlbrock), MPI for Plant Breeding Research, Max Planck Society, ou_1113566              

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 Abstract: PCR amplifications using tomato DNA and degenerate oligonucleotide primers allowed identification of a new putative nitrate transporter, termed NRT2;3. Its sequence showed typical motifs of a high affinity nitrate transporter of the Major Facilitator Superfamily (MFS). The formation of its mRNA was positively controlled by nitrate, and negatively by ammonium, but not by glutamine. In situ hybridization experiments showed that this transporter was mainly expressed in rhizodermal cells. Results from expression studies with two other nitrate transporters, LeNRT1;1 and LeNRT2;1, were essentially in accord with data of the literature. In roots colonized by the arbuscular mycorrhizal fungus Glomus intraradices Sy167, transcript formation of NRT2;3 extended to the inner cortical cells where the fungal structures, arbuscules and vesicles, were concentrated. Northern analyses indicated that the expression of only NRT2;3 among the transporters assayed was higher in AMF colonized tomato roots than in non-colonized controls. AMF-colonization caused a significant expression of a nitrate reductase gene of G. intraradices. The results may mean that AMF-colonization positively affects nitrate uptake from soil and nitrate allocation to the plant partner, probably mediated preferentially by LeNRT2;3. In addition, part of the nitrate taken up is reduced by the fungal partner itself and may then be transferred, when in excess, as glutamine to the plant symbiotic partner.

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Language(s): eng - English
 Dates: 2002-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 127714
ISI: 000175895400015
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Title: Physiologia Plantarum
  Alternative Title : Physiol. Plant.
Source Genre: Journal
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Pages: - Volume / Issue: 115 (1) Sequence Number: - Start / End Page: 125 - 136 Identifier: ISSN: 0031-9317